AMCA PTC study materials
AMCA PTC study resources

AMCA PTC key terms

AMCA PTC · Get comfortable with the vocabulary.

Standard precautions

Infection control practices applied to every patient regardless of diagnosis, including hand hygiene, gloves, gowns, masks, and safe handling of sharps and body fluids.

Related: Personal protective equipment, Hand hygiene, Exposure control plan
Personal protective equipment

Barrier items such as gloves, gowns, masks, eye protection, and face shields worn to prevent contact with blood, body fluids, and infectious agents.

Related: Standard precautions, Transmission-based precautions
Hand hygiene

Cleaning hands with soap and water or an alcohol-based rub at specified moments, such as before and after patient contact and after glove removal, to reduce transmission of organisms.

Related: Standard precautions, Nosocomial infection
Transmission-based precautions

Additional precautions beyond standard precautions used for patients with known or suspected contagious conditions, categorized as contact, droplet, or airborne and matched to how the organism spreads.

Related: Standard precautions, Personal protective equipment
Exposure control plan

A written employer program describing how workers are protected from bloodborne pathogens, including engineering controls, work practice controls, hepatitis B vaccination, training, and post-exposure procedures.

Related: Standard precautions, Post-exposure prophylaxis, Needlestick injury
Post-exposure prophylaxis

Medical treatment, such as antiviral medication or immune globulin, given after a possible exposure to an infectious agent to reduce the chance of developing infection.

Related: Exposure control plan, Needlestick injury
Needlestick injury

An accidental puncture of the skin by a used needle or other sharp, which must be reported immediately so the source can be evaluated and prophylaxis considered.

Related: Sharps container, Post-exposure prophylaxis, Exposure control plan
Sharps container

A puncture-resistant, closable, labeled receptacle used to discard needles, lancets, and other contaminated sharps immediately after use to prevent injury.

Related: Needlestick injury, Biohazard waste
Biohazard waste

Material contaminated with blood or other potentially infectious substances that must be segregated, labeled, and disposed of according to facility and regulatory rules.

Related: Sharps container, Standard precautions
Nosocomial infection

An infection a patient acquires during care in a health care setting that was not present or incubating at the time of admission.

Related: Hand hygiene, Transmission-based precautions
Chain of infection

The sequence by which infection spreads: a pathogen, a reservoir, a portal of exit, a mode of transmission, a portal of entry, and a susceptible host; breaking any link prevents spread.

Related: Standard precautions, Hand hygiene
Order of draw

The standardized sequence for filling blood collection tubes so that additive carryover between tubes does not alter test results, beginning with sterile culture tubes and ending with tubes containing additives that affect coagulation testing.

Related: Additive, Evacuated tube system, Clot activator
Additive

A substance placed inside a collection tube, such as an anticoagulant, clot activator, or separator gel, that preserves or prepares the sample for a specific laboratory test.

Related: Order of draw, Anticoagulant, Clot activator
Anticoagulant

A chemical additive, such as EDTA or sodium citrate, that prevents blood from clotting so cellular or plasma-based tests can be performed.

Related: Additive, Order of draw, Clot activator
Clot activator

An additive, often silica or a thrombin-based agent, that speeds formation of a clot so serum can be separated from cells after centrifugation.

Related: Additive, Serum separator tube, Order of draw
Serum separator tube

A tube containing clot activator and a gel barrier that, after centrifugation, separates serum from cells for chemistry and other serum-based testing.

Related: Clot activator, Centrifugation
Evacuated tube system

A closed collection method in which a needle and holder are used with tubes that draw a preset volume by vacuum, reducing exposure risk and standardizing fill.

Related: Order of draw, Butterfly needle, Hemolysis
Butterfly needle

A small-gauge winged needle attached to flexible tubing, used for fragile or small veins and for pediatric or difficult draws.

Related: Evacuated tube system, Venipuncture
Tourniquet

A strap applied above the puncture site to make veins more visible and firm; it should be released promptly because prolonged use alters blood chemistry and causes stasis.

Related: Venipuncture, Hemoconcentration
Hemoconcentration

An increase in the concentration of cells and large molecules in blood caused by fluid leaving the vessels, often from leaving a tourniquet on too long or from prolonged fist pumping.

Related: Tourniquet, Hemolysis
Hemolysis

Rupture of red blood cells that releases hemoglobin into the specimen, often from rough handling, small needles, or vigorous mixing, and can make results unreliable.

Related: Hemoconcentration, Centrifugation, Specimen rejection
Venipuncture

Entry into a vein with a needle to withdraw blood for laboratory testing or donation.

Related: Tourniquet, Butterfly needle, Palpation
Capillary puncture

Collection of a small blood sample by pricking the skin, most often the side of a fingertip or the heel of an infant, for tests that need only a drop or two.

Related: Lancet, Microcollection container, Venipuncture
Lancet

A small, sterile, single-use blade used to make a controlled puncture in the skin for capillary blood collection.

Related: Capillary puncture, Microcollection container
Microcollection container

A small tube or vial, often with a scoop or capillary tip, used to collect and hold tiny blood volumes obtained by skin puncture.

Related: Capillary puncture, Lancet
Palpation

Using the fingertips to feel a vein's location, depth, direction, and resilience before puncture, which helps select the best site and avoid arteries or damaged vessels.

Related: Venipuncture, Site selection
Site selection

Choosing the puncture location by considering vein condition, patient anatomy, prior draws, and areas to avoid such as scars, hematomas, or arms with intravenous lines or dialysis access.

Related: Palpation, Hemolysis
Patient identification

Verification of a patient's identity using at least two independent identifiers, such as full name and date of birth, plus matching to the requisition before any specimen is collected.

Related: Requisition, Specimen labeling, Informed consent
Requisition

The order or form that specifies the tests requested, patient information, and collection requirements; it must be checked against the patient and the labeled specimens.

Related: Patient identification, Specimen labeling
Specimen labeling

Applying identifying information to a specimen container at the bedside immediately after collection, including patient name, unique identifier, date, time, and collector, so the sample can be traced.

Related: Patient identification, Specimen rejection
Informed consent

A patient's voluntary agreement to a procedure after being told its purpose, what will happen, risks, and alternatives, given without coercion and documented as required.

Related: Patient identification, Confidentiality
Confidentiality

The ethical and legal duty to protect a patient's health information from unauthorized disclosure, sharing it only with those who need it for care or as permitted by law.

Related: Informed consent, Protected health information
Protected health information

Individually identifiable health data, in any form, that is protected by privacy rules and must be handled, stored, and transmitted securely.

Related: Confidentiality, Requisition
Scope of practice

The tasks and responsibilities a health care worker is legally and professionally permitted to perform based on training, certification, and state or employer rules.

Related: Standard of care, Informed consent
Standard of care

The level and type of care a reasonably competent professional with similar training would provide in similar circumstances, used to judge whether actions were appropriate.

Related: Scope of practice, Negligence
Negligence

Failure to meet the standard of care, resulting in harm, established by showing a duty, a breach, causation, and damages.

Related: Standard of care, Scope of practice
Centrifugation

Spinning a specimen at a controlled speed and time to separate components such as serum or plasma from cells, following the test's requirements for speed and duration.

Related: Serum separator tube, Hemolysis, Specimen rejection
Specimen rejection

Refusal of a sample for testing because it is mislabeled, hemolyzed, clotted, insufficient, improperly stored, or otherwise unsuitable, requiring documentation and often recollection.

Related: Specimen labeling, Hemolysis, Chain of custody
Chain of custody

A documented, unbroken record of who handled a specimen and when, used when results may have legal consequences, such as forensic or workplace drug testing.

Related: Specimen labeling, Specimen rejection
Fasting specimen

A sample collected after a required period without food or drink except water, because eating can change results such as glucose or lipid levels.

Related: Site selection, Requisition