ASCP PBT study materials
ASCP PBT study resources

ASCP PBT study plan

ASCP PBT · Give each week a clear focus.

8 weeks · About 6 hours per week

Week 1

Phlebotomy

  • List the order of draw for capillary and venous specimens and explain the rationale for each position

  • Identify the appropriate tube, additive, and anticoagulant for common tests (CBC, PT/INR, chemistry panel, blood bank type and screen)

  • Describe proper patient identification, site selection, and venipuncture technique for adult and pediatric patients

  • Recognize and correct common preanalytical errors (hemolysis, short draw, wrong tube, clotted specimen)

Your checkpoint

Create a one-page order-of-draw and tube-selection reference card and score at least 85% on a 30-question self-quiz covering phlebotomy procedures and preanalytical errors.

Week 2

Phlebotomy & Laboratory Administration

  • Explain safety protocols including PPE, sharps disposal, and post-exposure prophylaxis

  • Outline the chain of custody and special handling requirements for forensic and legal specimens

  • Describe quality assurance indicators for phlebotomy (e.g., hemolysis rate, redraw rate, patient wait time)

  • Identify regulatory agencies (CLIA, CAP, TJC) and their roles in laboratory accreditation and personnel standards

Your checkpoint

Draft a 1-page quality-monitoring plan for a phlebotomy service that includes two measurable indicators and a corrective-action workflow.

Week 3

Hematology

  • Differentiate reference ranges and morphological features of red blood cells, white blood cells, and platelets on a stained smear

  • Calculate and interpret RBC indices (MCV, MCH, MCHC) and reticulocyte counts

  • Classify anemias (microcytic, normocytic, macrocytic) based on laboratory data and explain underlying mechanisms

  • Describe the laboratory approach to diagnosing leukemia and lymphoma, including flow cytometry and cytochemical stains

Your checkpoint

Build a 10-slide anemia-classification decision tree with at least three laboratory parameters per branch and pass a 25-question hematology quiz with 80% or higher.

Week 4

Coagulation

  • Explain the coagulation cascade, including the intrinsic, extrinsic, and common pathways, and the role of vitamin K

  • Interpret PT/INR, aPTT, thrombin time, and fibrinogen results in the context of bleeding and thrombotic disorders

  • Describe specimen collection and processing requirements for coagulation testing (tube type, fill volume, time and temperature limits)

  • Identify causes and corrective actions for prolonged aPTT and prolonged PT

Your checkpoint

Complete a case-based worksheet of 10 coagulation scenarios with correct interpretation and next-step testing for at least 8 of 10 cases.

Week 5

Clinical Chemistry

  • Interpret chemistry panels (basic metabolic, hepatic, lipid, thyroid) and identify critical values requiring immediate notification

  • Explain the principles and interferences of common analytical methods (spectrophotometry, ion-selective electrodes, immunoassays)

  • Calculate and interpret creatinine clearance, anion gap, and osmolality

  • Describe quality control rules (Westgard) and troubleshooting for out-of-control chemistry results

Your checkpoint

Create a critical-value call list with at least 10 analytes and their thresholds, and correctly interpret 15 chemistry panel cases with 80% accuracy.

Week 6

Blood Banking

  • Explain ABO and Rh blood group systems, including expected reactions in forward and reverse typing

  • Describe the antibody detection (screen) and identification process, including panel cells and enzyme techniques

  • Outline pretransfusion testing, compatibility testing, and the significance of a positive antibody screen

  • Identify causes of hemolytic transfusion reactions and the laboratory's role in investigation

Your checkpoint

Complete three ABO/Rh discrepancy case studies with correct resolution and pass a 20-question blood bank quiz with 80% or higher.

Week 7

Immunology & Microbiology

  • Explain the principles of serological testing (agglutination, ELISA, immunofluorescence) and interpret qualitative and quantitative results

  • Describe the immune response, antibody classes, and the significance of IgM versus IgG in infectious disease and blood banking

  • Differentiate bacterial, viral, fungal, and parasitic pathogens by specimen type and laboratory method

  • Interpret Gram stain, culture, and susceptibility results and explain quality control for microbiology media and reagents

Your checkpoint

Build a comparison table of at least eight serological methods and their clinical applications, and correctly answer 20 mixed immunology/microbiology questions with 80% accuracy.

Week 8

Phlebotomy & Blood Banking & Clinical Chemistry & Coagulation & Hematology & Immunology & Laboratory Administration & Microbiology

  • Review high-yield concepts from all eight domains using spaced retrieval practice

  • Complete two full-length timed practice exams under test-like conditions

  • Analyze missed questions by domain and create a targeted review list of weak areas

  • Rehearse critical-value reporting and safety scenarios for the phlebotomy and administration domains

Your checkpoint

Score at least 80% on two full-length practice exams and produce a final one-page summary sheet of the top 20 weak concepts with corrected explanations.

Milestones

Week 2: Score 85% or higher on a cumulative 40-question quiz covering Phlebotomy and Laboratory Administration, and submit the quality-monitoring plan.

Week 4: Score 80% or higher on a cumulative 50-question quiz covering Phlebotomy, Laboratory Administration, Hematology, and Coagulation, and complete the coagulation case worksheet.

Week 6: Score 80% or higher on a cumulative 60-question quiz covering all domains studied to date, and resolve all three blood bank discrepancy cases correctly.

Week 8: Score 80% or higher on two full-length practice exams and submit the final weak-concept summary sheet.