AATO Practice Exam study materials
AATO Practice Exam study resources

AATO Practice Exam key terms

AATO Practice Exam · Get comfortable with the vocabulary.

Animal Welfare Act (AWA)

The primary U.S. federal law that sets minimum standards for the humane care, handling, treatment, and transport of certain animals, including many used in research, exhibition, and commercial breeding.

Related: USDA APHIS Animal Care, Attending Veterinarian, Institutional Animal Care and Use Committee (IACUC)
USDA APHIS Animal Care

The federal agency unit responsible for enforcing the Animal Welfare Act through licensing, registration, inspections, and investigation of regulated animal facilities.

Related: Animal Welfare Act (AWA), Regulated Animal
Animal Welfare Regulations (9 CFR)

The detailed federal rules in Title 9 of the Code of Federal Regulations that translate the Animal Welfare Act into specific, enforceable care and housing requirements.

Related: Animal Welfare Act (AWA), Minimum Standards
Public Health Service (PHS) Policy

Federal policy requiring humane care of vertebrate animals used in federally funded research, applying to all vertebrate species and tied to the Guide for the Care and Use of Laboratory Animals.

Related: The Guide (Guide for the Care and Use of Laboratory Animals), Institutional Animal Care and Use Committee (IACUC)
The Guide (Guide for the Care and Use of Laboratory Animals)

A widely adopted reference text providing science-based recommendations for animal care, housing, husbandry, veterinary care, and facility design in research settings.

Related: Public Health Service (PHS) Policy, Attending Veterinarian
Institutional Animal Care and Use Committee (IACUC)

A facility committee that reviews and approves animal use protocols, inspects facilities, and oversees the institution's animal care and use program to ensure regulatory compliance and animal welfare.

Related: Animal Use Protocol, Attending Veterinarian, Animal Welfare Act (AWA)
Animal Use Protocol

A written, reviewed plan describing why animals are used, the species and numbers involved, procedures, housing, analgesia, and euthanasia, which must be approved before work begins.

Related: Institutional Animal Care and Use Committee (IACUC), Three Rs
Regulated Animal

An animal species covered by the Animal Welfare Act, such as dogs, cats, nonhuman primates, guinea pigs, hamsters, rabbits, and certain other mammals, but excluding purpose-bred mice and rats.

Related: Animal Welfare Act (AWA), USDA APHIS Animal Care
Three Rs

The ethical principles of replacement (avoiding animal use where possible), reduction (using fewer animals), and refinement (minimizing pain and distress) that guide humane study design.

Related: Animal Use Protocol, Environmental Enrichment, Ethical Review
Attending Veterinarian

The licensed veterinarian with authority and responsibility for the health, welfare, and medical care of animals at a facility, including oversight of analgesia, anesthesia, and euthanasia.

Related: Veterinary-Client-Patient Relationship, Institutional Animal Care and Use Committee (IACUC), Euthanasia
Occupational Health and Safety Program

A facility program that identifies workplace hazards such as allergens, zoonoses, and injuries, and provides training, monitoring, and protective measures for personnel working with animals.

Related: Zoonosis, Personal Protective Equipment (PPE), Needlestick Injury
Standard Operating Procedure (SOP)

A written, approved set of step-by-step instructions that staff follow to perform routine tasks consistently, such as feeding, cleaning, or health checks.

Related: Recordkeeping Requirement, Daily Observation Log
Recordkeeping Requirement

The regulatory obligation to create and retain accurate records such as acquisition and disposition logs, health records, and inspection reports for a defined retention period.

Related: Standard Operating Procedure (SOP), Daily Observation Log, Animal Use Protocol
Daily Observation Log

A record where staff document each animal's condition, food and water intake, behavior, and any abnormalities so that health changes are detected and tracked over time.

Related: Recordkeeping Requirement, Standard Operating Procedure (SOP)
Chain of Custody

A documented, unbroken record of who handled a sample, animal, or item and when, used to preserve traceability for medical, legal, or investigative purposes.

Related: Recordkeeping Requirement, Necropsy
Zoonosis

An infectious disease that can be transmitted from animals to humans, such as rabies, salmonellosis, or ringworm, requiring specific precautions for people working with animals.

Related: Biosecurity, Personal Protective Equipment (PPE), Occupational Health and Safety Program
Biosecurity

Practices and measures designed to prevent the introduction and spread of infectious agents into and within an animal facility, including access control, sanitation, and traffic flow.

Related: Quarantine, Sanitation, Zoonosis
Quarantine

The separation and isolation of newly arrived or potentially exposed animals for a defined period to prevent the spread of disease and to allow health monitoring.

Related: Biosecurity, Isolation Room
Isolation Room

A dedicated, controlled space used to house animals known or suspected to be infectious, with restricted access and specific cleaning and waste-handling procedures.

Related: Quarantine, Biosecurity, Sanitation
Sanitation

The process of cleaning and disinfecting surfaces, cages, and equipment to reduce or eliminate infectious agents and maintain hygienic conditions in animal housing areas.

Related: Disinfectant, Biosecurity, Isolation Room
Disinfectant

A chemical agent applied to inanimate surfaces to destroy or inactivate most disease-causing microorganisms, chosen for effectiveness against the target pathogens and for safety.

Related: Sanitation, Personal Protective Equipment (PPE)
Personal Protective Equipment (PPE)

Items such as gloves, gowns, masks, eye protection, and shoe covers worn to protect personnel from biological, chemical, and physical hazards while working with animals.

Related: Zoonosis, Occupational Health and Safety Program, Needlestick Injury
Needlestick Injury

An accidental puncture of the skin by a contaminated needle or sharp, a common occupational hazard that requires immediate first aid, reporting, and medical follow-up.

Related: Sharps Container, Occupational Health and Safety Program, Personal Protective Equipment (PPE)
Sharps Container

A rigid, puncture-resistant, labeled container used to safely dispose of needles, scalpel blades, and other sharp items to prevent injury and exposure to bloodborne pathogens.

Related: Needlestick Injury
Environmental Enrichment

Structured additions to an animal's environment, such as nesting materials, toys, social housing, or foraging opportunities, that promote natural behaviors and psychological well-being.

Related: Social Housing, Three Rs, Stereotypy
Social Housing

Housing compatible animals together so they can interact, which supports natural social behavior and well-being, unless veterinary or study reasons require individual housing.

Related: Environmental Enrichment, Single Housing Justification
Single Housing Justification

Documented reasons, reviewed and approved where required, explaining why an animal must be housed alone for health, welfare, or scientific reasons.

Related: Social Housing, Daily Observation Log
Acclimation

The period after arrival during which animals adjust to new housing, personnel, and routines, allowing stress to settle and baseline health to be assessed before procedures begin.

Related: Quarantine, Handling
Handling

The techniques used to safely restrain, lift, and move animals with minimal stress and risk of injury to the animal and the handler.

Related: Acclimation, Restraint
Restraint

The use of manual or mechanical methods to limit an animal's movement for examination or procedures, applied with the least force and shortest duration necessary.

Related: Handling, Anesthesia
Stereotypy

A repetitive, invariant behavior such as pacing or bar-biting that often signals poor welfare, frustration, or an environment that does not meet the animal's behavioral needs.

Related: Environmental Enrichment, Daily Observation Log
Anesthesia

The controlled use of drugs to produce loss of sensation or consciousness, preventing pain during procedures and requiring monitoring of vital signs throughout.

Related: Analgesia, Restraint, Attending Veterinarian
Analgesia

The use of drugs to relieve or prevent pain, which must be provided when procedures cause more than momentary or slight pain or distress.

Related: Anesthesia, Pain and Distress Classification, Attending Veterinarian
Pain and Distress Classification

The category assigned to a procedure describing its expected level of pain or distress, used by oversight bodies to justify humane endpoints and appropriate pain relief.

Related: Analgesia, Humane Endpoint
Humane Endpoint

A defined point at which an experiment or observation is stopped and the animal is treated or euthanized to prevent unnecessary pain or distress.

Related: Pain and Distress Classification, Euthanasia, Animal Use Protocol
Euthanasia

The humane induction of death using methods that produce rapid unconsciousness and death without pain, distress, or fear, following accepted guidelines.

Related: Humane Endpoint, Attending Veterinarian, Necropsy
Necropsy

A systematic postmortem examination of an animal's body to determine the cause of death or the nature of disease, with findings documented in the health record.

Related: Euthanasia, Chain of Custody
Veterinary-Client-Patient Relationship

The professional relationship in which a veterinarian assumes responsibility for an animal's care with the owner's consent, required for legitimate prescribing and treatment.

Related: Attending Veterinarian, Informed Consent
Informed Consent

The process of clearly explaining procedures, risks, and alternatives so that a person can voluntarily agree to participate or to allow animal use, without coercion.

Related: Ethical Review, Veterinary-Client-Patient Relationship
Ethical Review

A structured evaluation of proposed animal use against welfare, scientific merit, and regulatory criteria to ensure the work is justified and humane.

Related: Three Rs, Informed Consent, Institutional Animal Care and Use Committee (IACUC)
Conflict of Interest

A situation where personal, financial, or other interests could improperly influence professional judgment, which must be disclosed and managed to preserve integrity.

Related: Ethical Review, Informed Consent
Minimum Standards

The baseline care requirements set by law or policy, such as space, food, water, shelter, and veterinary care, below which an animal facility may not operate.

Related: Animal Welfare Regulations (9 CFR), The Guide (Guide for the Care and Use of Laboratory Animals)