AATO Practice Exam key terms
AATO Practice Exam · Get comfortable with the vocabulary.
- Animal Welfare Act (AWA)
- Related: USDA APHIS Animal Care, Attending Veterinarian, Institutional Animal Care and Use Committee (IACUC)
The primary U.S. federal law that sets minimum standards for the humane care, handling, treatment, and transport of certain animals, including many used in research, exhibition, and commercial breeding.
- USDA APHIS Animal Care
- Related: Animal Welfare Act (AWA), Regulated Animal
The federal agency unit responsible for enforcing the Animal Welfare Act through licensing, registration, inspections, and investigation of regulated animal facilities.
- Animal Welfare Regulations (9 CFR)
- Related: Animal Welfare Act (AWA), Minimum Standards
The detailed federal rules in Title 9 of the Code of Federal Regulations that translate the Animal Welfare Act into specific, enforceable care and housing requirements.
- Public Health Service (PHS) Policy
- Related: The Guide (Guide for the Care and Use of Laboratory Animals), Institutional Animal Care and Use Committee (IACUC)
Federal policy requiring humane care of vertebrate animals used in federally funded research, applying to all vertebrate species and tied to the Guide for the Care and Use of Laboratory Animals.
- The Guide (Guide for the Care and Use of Laboratory Animals)
- Related: Public Health Service (PHS) Policy, Attending Veterinarian
A widely adopted reference text providing science-based recommendations for animal care, housing, husbandry, veterinary care, and facility design in research settings.
- Institutional Animal Care and Use Committee (IACUC)
- Related: Animal Use Protocol, Attending Veterinarian, Animal Welfare Act (AWA)
A facility committee that reviews and approves animal use protocols, inspects facilities, and oversees the institution's animal care and use program to ensure regulatory compliance and animal welfare.
- Animal Use Protocol
- Related: Institutional Animal Care and Use Committee (IACUC), Three Rs
A written, reviewed plan describing why animals are used, the species and numbers involved, procedures, housing, analgesia, and euthanasia, which must be approved before work begins.
- Regulated Animal
- Related: Animal Welfare Act (AWA), USDA APHIS Animal Care
An animal species covered by the Animal Welfare Act, such as dogs, cats, nonhuman primates, guinea pigs, hamsters, rabbits, and certain other mammals, but excluding purpose-bred mice and rats.
- Three Rs
- Related: Animal Use Protocol, Environmental Enrichment, Ethical Review
The ethical principles of replacement (avoiding animal use where possible), reduction (using fewer animals), and refinement (minimizing pain and distress) that guide humane study design.
- Attending Veterinarian
- Related: Veterinary-Client-Patient Relationship, Institutional Animal Care and Use Committee (IACUC), Euthanasia
The licensed veterinarian with authority and responsibility for the health, welfare, and medical care of animals at a facility, including oversight of analgesia, anesthesia, and euthanasia.
- Occupational Health and Safety Program
- Related: Zoonosis, Personal Protective Equipment (PPE), Needlestick Injury
A facility program that identifies workplace hazards such as allergens, zoonoses, and injuries, and provides training, monitoring, and protective measures for personnel working with animals.
- Standard Operating Procedure (SOP)
- Related: Recordkeeping Requirement, Daily Observation Log
A written, approved set of step-by-step instructions that staff follow to perform routine tasks consistently, such as feeding, cleaning, or health checks.
- Recordkeeping Requirement
- Related: Standard Operating Procedure (SOP), Daily Observation Log, Animal Use Protocol
The regulatory obligation to create and retain accurate records such as acquisition and disposition logs, health records, and inspection reports for a defined retention period.
- Daily Observation Log
- Related: Recordkeeping Requirement, Standard Operating Procedure (SOP)
A record where staff document each animal's condition, food and water intake, behavior, and any abnormalities so that health changes are detected and tracked over time.
- Chain of Custody
- Related: Recordkeeping Requirement, Necropsy
A documented, unbroken record of who handled a sample, animal, or item and when, used to preserve traceability for medical, legal, or investigative purposes.
- Zoonosis
- Related: Biosecurity, Personal Protective Equipment (PPE), Occupational Health and Safety Program
An infectious disease that can be transmitted from animals to humans, such as rabies, salmonellosis, or ringworm, requiring specific precautions for people working with animals.
- Biosecurity
- Related: Quarantine, Sanitation, Zoonosis
Practices and measures designed to prevent the introduction and spread of infectious agents into and within an animal facility, including access control, sanitation, and traffic flow.
- Quarantine
- Related: Biosecurity, Isolation Room
The separation and isolation of newly arrived or potentially exposed animals for a defined period to prevent the spread of disease and to allow health monitoring.
- Isolation Room
- Related: Quarantine, Biosecurity, Sanitation
A dedicated, controlled space used to house animals known or suspected to be infectious, with restricted access and specific cleaning and waste-handling procedures.
- Sanitation
- Related: Disinfectant, Biosecurity, Isolation Room
The process of cleaning and disinfecting surfaces, cages, and equipment to reduce or eliminate infectious agents and maintain hygienic conditions in animal housing areas.
- Disinfectant
- Related: Sanitation, Personal Protective Equipment (PPE)
A chemical agent applied to inanimate surfaces to destroy or inactivate most disease-causing microorganisms, chosen for effectiveness against the target pathogens and for safety.
- Personal Protective Equipment (PPE)
- Related: Zoonosis, Occupational Health and Safety Program, Needlestick Injury
Items such as gloves, gowns, masks, eye protection, and shoe covers worn to protect personnel from biological, chemical, and physical hazards while working with animals.
- Needlestick Injury
- Related: Sharps Container, Occupational Health and Safety Program, Personal Protective Equipment (PPE)
An accidental puncture of the skin by a contaminated needle or sharp, a common occupational hazard that requires immediate first aid, reporting, and medical follow-up.
- Sharps Container
- Related: Needlestick Injury
A rigid, puncture-resistant, labeled container used to safely dispose of needles, scalpel blades, and other sharp items to prevent injury and exposure to bloodborne pathogens.
- Environmental Enrichment
- Related: Social Housing, Three Rs, Stereotypy
Structured additions to an animal's environment, such as nesting materials, toys, social housing, or foraging opportunities, that promote natural behaviors and psychological well-being.
- Social Housing
- Related: Environmental Enrichment, Single Housing Justification
Housing compatible animals together so they can interact, which supports natural social behavior and well-being, unless veterinary or study reasons require individual housing.
- Single Housing Justification
- Related: Social Housing, Daily Observation Log
Documented reasons, reviewed and approved where required, explaining why an animal must be housed alone for health, welfare, or scientific reasons.
- Acclimation
- Related: Quarantine, Handling
The period after arrival during which animals adjust to new housing, personnel, and routines, allowing stress to settle and baseline health to be assessed before procedures begin.
- Handling
- Related: Acclimation, Restraint
The techniques used to safely restrain, lift, and move animals with minimal stress and risk of injury to the animal and the handler.
- Restraint
- Related: Handling, Anesthesia
The use of manual or mechanical methods to limit an animal's movement for examination or procedures, applied with the least force and shortest duration necessary.
- Stereotypy
- Related: Environmental Enrichment, Daily Observation Log
A repetitive, invariant behavior such as pacing or bar-biting that often signals poor welfare, frustration, or an environment that does not meet the animal's behavioral needs.
- Anesthesia
- Related: Analgesia, Restraint, Attending Veterinarian
The controlled use of drugs to produce loss of sensation or consciousness, preventing pain during procedures and requiring monitoring of vital signs throughout.
- Analgesia
- Related: Anesthesia, Pain and Distress Classification, Attending Veterinarian
The use of drugs to relieve or prevent pain, which must be provided when procedures cause more than momentary or slight pain or distress.
- Pain and Distress Classification
- Related: Analgesia, Humane Endpoint
The category assigned to a procedure describing its expected level of pain or distress, used by oversight bodies to justify humane endpoints and appropriate pain relief.
- Humane Endpoint
- Related: Pain and Distress Classification, Euthanasia, Animal Use Protocol
A defined point at which an experiment or observation is stopped and the animal is treated or euthanized to prevent unnecessary pain or distress.
- Euthanasia
- Related: Humane Endpoint, Attending Veterinarian, Necropsy
The humane induction of death using methods that produce rapid unconsciousness and death without pain, distress, or fear, following accepted guidelines.
- Necropsy
- Related: Euthanasia, Chain of Custody
A systematic postmortem examination of an animal's body to determine the cause of death or the nature of disease, with findings documented in the health record.
- Veterinary-Client-Patient Relationship
- Related: Attending Veterinarian, Informed Consent
The professional relationship in which a veterinarian assumes responsibility for an animal's care with the owner's consent, required for legitimate prescribing and treatment.
- Informed Consent
- Related: Ethical Review, Veterinary-Client-Patient Relationship
The process of clearly explaining procedures, risks, and alternatives so that a person can voluntarily agree to participate or to allow animal use, without coercion.
- Ethical Review
- Related: Three Rs, Informed Consent, Institutional Animal Care and Use Committee (IACUC)
A structured evaluation of proposed animal use against welfare, scientific merit, and regulatory criteria to ensure the work is justified and humane.
- Conflict of Interest
- Related: Ethical Review, Informed Consent
A situation where personal, financial, or other interests could improperly influence professional judgment, which must be disclosed and managed to preserve integrity.
- Minimum Standards
- Related: Animal Welfare Regulations (9 CFR), The Guide (Guide for the Care and Use of Laboratory Animals)
The baseline care requirements set by law or policy, such as space, food, water, shelter, and veterinary care, below which an animal facility may not operate.