DANB ICE study materials
DANB ICE study resources

DANB ICE cheat sheet

DANB ICE · Keep the key ideas within reach.

Quick reference

Infection Control Concepts and Standard Precautions

  • Chain of infection: infectious agent → reservoir → portal of exit → mode of transmission → portal of entry → susceptible host; breaking any one link prevents transmission.

  • Standard precautions apply to every patient regardless of known diagnosis, whenever contact with blood, all body fluids and secretions (except sweat), nonintact skin, or mucous membranes is possible.

  • Alcohol-based hand rub: apply and rub until dry, about 20–30 seconds; use soap and water for 40–60 seconds when hands are visibly soiled or after exposure to spore-formers (C. difficile, norovirus).

  • PPE sequence: gown → mask/respirator → eye protection → gloves; remove in reverse order with gloves last, then perform hand hygiene.

  • Droplet precautions: particles >5 µm traveling about 3 ft - mask plus eye protection for close contact. Airborne precautions: particles <5 µm that stay suspended - N95, negative-pressure room, door closed.

  • Sharps rule: never recap, bend, break, or hand-remove needles; place immediately in a closable, puncture-resistant, leakproof, biohazard-labeled container.

  • Aerosol-generating procedures (ultrasonic scaler, air polisher, high-speed handpiece, air-water syringe) require high-volume evacuation plus a surgical mask and face shield.

  • Gloves never substitute for hand hygiene; perform hand hygiene before donning and immediately after removing gloves.

  • Key bloodborne pathogens in dentistry: HBV (most transmissible; stable on dried surfaces ≥7 days), HCV, and HIV; HBV is vaccine-preventable.

Quick reference

Environmental Asepsis and Surface Management

  • Spaulding classification: critical = enters sterile tissue, bone, or vasculature → sterilize; semicritical = contacts mucous membrane or nonintact skin → sterilize or high-level disinfect; noncritical = intact skin only → low-level disinfect or barrier.

  • Clinical contact surfaces (light handles, chair switches, bracket trays, drawer pulls) receive a barrier or clean-and-disinfect with an EPA-registered intermediate-level (tuberculocidal) disinfectant between patients.

  • Housekeeping surfaces (floors, walls, sinks) require detergent and water or a low-level disinfectant; disinfect only after visible contamination has been cleaned away.

  • Disinfectant efficacy depends on contact time: the surface must stay visibly wet for the full manufacturer-labeled time to achieve the claimed kill.

  • A compromised barrier is treated as an uncovered surface: remove barrier, clean, disinfect, then place a new barrier.

  • Dental unit waterlines: flush 20–30 seconds between patients and at the start of the day; an EPA-registered treatment keeps heterotrophic plate counts ≤500 CFU/mL for nonsurgical care.

  • Suction/evacuation lines: flush with water, then draw the manufacturer-specified detergent/disinfectant through the line; use a disposable single-patient suction tip.

  • Regulated medical waste = blood-saturated or dripping items and sharps → red bag or rigid biohazard container; saliva-soaked gauze, gloves, and masks are nonregulated.

  • Radiographic sensors and phosphor plates: fresh barrier for each patient, disinfect after barrier removal, and never set a contaminated item on the exposure switch.

Quick reference

Sterilization and Disinfection of Instruments and Equipment

  • Sterilization destroys all microbial life including spores; disinfection reduces pathogens but may spare spores - critical and semicritical items are heat-sterilized whenever the item tolerates it.

  • Sequence: transport in a closed container → clean (ultrasonic with enzymatic detergent, rinse, dry) → inspect → package and label → sterilize → monitor → store in a closed cabinet.

  • Steam autoclave: gravity displacement about 121 °C (250 °F) at 15–20 psi for 30 minutes, or 132 °C (270 °F) for 4 minutes; follow the sterilizer manufacturer's validated cycle.

  • Load configuration: hinged instruments open, chamber not overloaded, packages not touching chamber walls, mixed metal/porous loads only as the manufacturer permits.

  • Three monitoring levels: mechanical (gauge/printout), chemical (external process indicator plus internal indicator), biological (spore test) - only the biological indicator proves sterilization.

  • Steam BI organism is Geobacillus stearothermophilus; dry-heat BI organism is Bacillus atrophaeus. Run at least weekly and with every implant load; a failed BI triggers recall to the last passing load.

  • High-level disinfection of heat-sensitive semicritical items: glutaraldehyde 2.0–3.4% (≥20 minutes at 20–25 °C) or ortho-phthalaldehyde; test minimum effective concentration and ventilate the area.

  • Single-use disposable items (needles, blades, plastic matrix bands, designated burs) are never reprocessed.

  • Package label must carry date of sterilization, sterilizer/cycle identifier, and preparer; event-related shelf life applies only while the package remains dry and intact.

Quick reference

Occupational Safety and Infection Control Administration

  • OSHA Bloodborne Pathogens Standard (29 CFR 1910.1030) requires a written Exposure Control Plan, reviewed and updated at least annually and whenever tasks or technology change.

  • HBV vaccination: offered free within 10 working days of assignment to exposure-prone duties; declination must be in writing, and the vaccine may be accepted later at no cost.

  • Post-exposure protocol: wash the wound with soap and water without squeezing, flush splashes, report immediately, document source and route, and obtain evaluation/post-exposure prophylaxis promptly.

  • Training is required at initial assignment and annually; training records are kept 3 years, medical and exposure records are kept for duration of employment plus 30 years, both confidential.

  • PPE is employer-provided at no cost, including laundering of reusable gowns; gloves never substitute for hand hygiene or engineering controls.

  • Hazard Communication Standard requires an accessible Safety Data Sheet for every hazardous chemical on site (disinfectants, glutaraldehyde, amalgam, etchants).

  • Regulated waste program: labeled biohazard containers, sharps containers closed and replaced at three-quarters full, waste manifests retained per state law.

  • Respiratory protection for suspected or confirmed tuberculosis and aerosol-generating procedures: annually fit-tested N95, negative-pressure room, door closed, symptom screening.

  • Employees with exudative or weeping lesions on the hands are reassigned away from direct patient contact until the condition resolves.