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Question 1Survey of the Natural Sciences
Which organelle is the principal site of ATP production by oxidative phosphorylation in a eukaryotic cell?
- Smooth endoplasmic reticulum
- Golgi apparatus
- Mitochondrion Correct answer.
- Lysosome
Explanation
The inner mitochondrial membrane houses the electron transport chain and ATP synthase, which couple proton gradient dissipation to ATP synthesis. The other listed organelles handle protein and lipid processing or macromolecule degradation.
Question 2Survey of the Natural Sciences
How many moles of solute are present in 2.0 L of a 0.50 M aqueous solution?
- 0.25 mol
- 2.0 mol
- 4.0 mol
- 1.0 mol Correct answer.
Explanation
Molarity is defined as moles of solute per liter of solution, so moles = M × V = 0.50 mol/L × 2.0 L = 1.0 mol.
Question 3Survey of the Natural Sciences
Which functional group is present in every carboxylic acid?
- Hydroxyl group
- Carbonyl group
- Ester linkage
- Carboxyl group Correct answer.
Explanation
A carboxylic acid contains a carboxyl group, in which a carbonyl carbon is bonded to a hydroxyl oxygen. The combined arrangement produces the acidic O–H proton that defines the class.
Question 4Survey of the Natural Sciences
In a cross between two individuals heterozygous at a single locus (Aa × Aa), what fraction of offspring is expected to express the recessive phenotype?
- 1/4 Correct answer.
- 3/4
- 0
- 1/2
Explanation
Aa × Aa produces a genotypic ratio of 1 AA : 2 Aa : 1 aa, and only the homozygous recessive class expresses the recessive trait, giving one quarter of the offspring.
Question 5Survey of the Natural Sciences
Which element has the ground-state electron configuration 1s² 2s² 2p⁶ 3s² 3p⁶?
- Sulfur
- Chlorine
- Argon Correct answer.
- Potassium
Explanation
Summing the superscripts gives 2 + 2 + 6 + 2 + 6 = 18 electrons, which is the atomic number of argon. A completely filled 3p subshell marks the end of period 3, characteristic of a noble gas.
Question 6Survey of the Natural Sciences
Which vessel carries oxygenated blood from the lungs to the heart?
- Pulmonary vein Correct answer.
- Aorta
- Superior vena cava
- Pulmonary artery
Explanation
Pulmonary veins return blood that has been oxygenated in the lungs to the left atrium. The pulmonary artery is the exception to the usual rule, carrying deoxygenated blood away from the heart toward the lungs.
Question 7Survey of the Natural Sciences
What is the hybridization of the carbon atom in methane?
- sp
- sp³d
- sp²
- sp³ Correct answer.
Explanation
The carbon in methane forms four equivalent sigma bonds with tetrahedral geometry, which requires mixing one s orbital with all three p orbitals to give four sp³ hybrids.
Question 8Survey of the Natural Sciences
Which intermolecular force is chiefly responsible for the unusually high boiling point of water?
- Dipole–dipole attractions
- Ion–dipole interactions
- London dispersion forces
- Hydrogen bonding Correct answer.
Explanation
Each water molecule can donate two hydrogen atoms to, and accept two from, neighboring molecules, producing an extensive three-dimensional hydrogen-bond network. Substantial energy is needed to break that network before molecules can enter the vapor phase.
Question 9Survey of the Natural Sciences
A competitive inhibitor is added to an enzyme-catalyzed reaction. Which kinetic change is expected?
- Km increases, Vmax unchanged Correct answer.
- Km unchanged, Vmax decreases
- Km decreases, Vmax decreases
- Km unchanged, Vmax increases
Explanation
A competitive inhibitor binds the active site reversibly, so its effect is overcome by raising substrate concentration. Vmax is therefore unchanged, while the apparent Km rises because more substrate is needed to reach half-maximal velocity.
Question 10Survey of the Natural Sciences
What is the pH of a 0.010 M aqueous solution of HCl at 25 °C?
- 1.0
- 12.0
- 2.0 Correct answer.
- 7.0
Explanation
HCl is a strong acid that dissociates essentially completely, so [H⁺] equals 1.0 × 10⁻² M. The pH is −log(1.0 × 10⁻²) = 2.0.
Question 11Survey of the Natural Sciences
Treating a primary alcohol with excess Jones reagent (CrO₃ in aqueous H₂SO₄) yields which product?
- An alkene
- A ketone
- A carboxylic acid Correct answer.
- An aldehyde only
Explanation
Aqueous acidic chromium(VI) oxidizes primary alcohols through the aldehyde to the carboxylic acid, since water readily converts the intermediate hydrate. Ketones can only arise from secondary alcohols, which have no hydrogen on the carbinol carbon.
Question 12Survey of the Natural Sciences
In the mitochondrial electron transport chain, which species serves as the final electron acceptor?
- NAD⁺
- O₂ Correct answer.
- Pyruvate
- FADH₂
Explanation
Complex IV transfers electrons to molecular oxygen, which is reduced to water, allowing the chain to keep running. NAD⁺ and FADH₂ act earlier as electron carriers that donate reducing equivalents to the chain.
Question 13Survey of the Natural Sciences
Which of the following ground-state atoms has the largest atomic radius?
- Magnesium
- Aluminum
- Sodium
- Potassium Correct answer.
Explanation
Atomic radius increases down a group because electrons occupy higher principal shells, and decreases across a period as effective nuclear charge rises. Potassium lies one period below sodium, so it is larger than all of the period-3 metals listed.
Question 14Survey of the Natural Sciences
A normal human somatic cell in the G2 phase contains how many sister chromatids?
- 92 Correct answer.
- 23
- 69
- 46
Explanation
DNA replication in S phase converts each of the 46 chromosomes into a pair of identical sister chromatids. The G2 cell therefore contains 46 × 2 = 92 chromatids.
Question 15Survey of the Natural Sciences
An SN2 reaction at a chiral secondary carbon typically proceeds with which stereochemical outcome?
- Complete retention of configuration
- Inversion of configuration Correct answer.
- Partial retention with some racemization
- No stereochemical change at all
Explanation
The nucleophile attacks the back lobe of the carbon–halogen sigma antibonding orbital while the leaving group departs from the opposite face. This backside displacement inverts the stereocenter, much like a windblown umbrella, whereas SN1 gives racemization.
Question 16Survey of the Natural Sciences
Which of the following aqueous solutes behaves as a weak electrolyte?
- Sodium chloride
- Glucose
- Hydrochloric acid
- Acetic acid Correct answer.
Explanation
Acetic acid ionizes only partially in water (Ka ≈ 1.8 × 10⁻⁵), so it produces few ions and conducts weakly. Strong electrolytes such as NaCl and HCl ionize completely, while glucose dissolves as neutral molecules.
Question 17Survey of the Natural Sciences
In a population at Hardy–Weinberg equilibrium, the frequency of a recessive allele is 0.2. What is the expected frequency of heterozygotes?
- 0.64
- 0.80
- 0.04
- 0.32 Correct answer.
Explanation
With q = 0.2, p = 1 − q = 0.8. The heterozygote frequency is 2pq = 2(0.8)(0.2) = 0.32.
Question 18Survey of the Natural Sciences
What is the approximate H–N–H bond angle in ammonia?
- 107° Correct answer.
- 120°
- 180°
- 90°
Explanation
Nitrogen in NH₃ has three bonding pairs and one lone pair, giving a trigonal pyramidal shape. The lone pair occupies more space than a bond pair, compressing the ideal tetrahedral angle of 109.5° to about 107°.
Question 19Survey of the Natural Sciences
Which carbocation is the most stable?
- (CH₃)₃C⁺ Correct answer.
- (CH₃)₂CH⁺
- CH₃⁺
- CH₃CH₂⁺
Explanation
Alkyl substituents stabilize a carbocation through inductive electron donation and hyperconjugation. The tert-butyl cation has three alkyl groups and is tertiary, making it the most stabilized of the four.
Question 20Survey of the Natural Sciences
Which hormone is released from the posterior pituitary?
- Thyroid-stimulating hormone
- Adrenocorticotropic hormone
- Antidiuretic hormone Correct answer.
- Prolactin
Explanation
The posterior pituitary stores and releases antidiuretic hormone and oxytocin, both synthesized by hypothalamic neurons. Thyroid-stimulating hormone, prolactin, and adrenocorticotropic hormone are anterior pituitary products.
Question 21Survey of the Natural Sciences
A woman who is a carrier of an X-linked recessive disorder has children with an unaffected man. What fraction of their daughters is expected to be carriers?
- 3/4
- 1/4
- 0
- 1/2 Correct answer.
Explanation
Every daughter inherits a normal X chromosome from her father, so her phenotype depends on which maternal X she receives. The carrier mother transmits the mutant allele half the time, making half of the daughters carriers.
Question 22Survey of the Natural Sciences
Given E°(Zn²⁺/Zn) = –0.76 V and E°(Ag⁺/Ag) = +0.80 V, what is E°cell for the galvanic cell Zn | Zn²⁺ || Ag⁺ | Ag?
- –0.04 V
- +1.56 V Correct answer.
- +0.04 V
- –1.56 V
Explanation
For a galvanic cell, E°cell = E°cathode − E°anode = 0.80 V − (−0.76 V) = +1.56 V. Silver is reduced at the cathode while zinc is oxidized at the anode, and the positive value confirms the reaction is spontaneous.
Question 23Survey of the Natural Sciences
Hydroboration–oxidation of 2-methyl-2-butene gives which compound as the major product?
- 2-methyl-1-butanol
- 2-methyl-2-butanol
- 3-methyl-2-butanol Correct answer.
- 3-methyl-1-butanol
Explanation
Borane adds so that boron ends up on the less substituted carbon of the double bond, and oxidation replaces boron with hydroxyl while retaining configuration. The result is anti-Markovnikov, syn addition of water, placing OH on C-3 and H on C-2.
Question 24Survey of the Natural Sciences
A woman affected by a mitochondrial DNA disorder has children with an unaffected man. Which pattern best describes the expected inheritance?
- Half of all children inherit it
- Only sons are affected
- All children inherit it Correct answer.
- Only daughters are affected
Explanation
Mitochondrial DNA is transmitted almost exclusively through the oocyte, so every child of an affected mother inherits her mitochondrial genome. Clinical expression can vary among offspring because of heteroplasmy, the mixture of mutant and normal mitochondrial genomes.
Question 25Survey of the Natural Sciences
Which 0.10 m aqueous solution has the highest boiling point?
- 0.10 m NaCl
- 0.10 m KCl
- 0.10 m glucose
- 0.10 m CaCl₂ Correct answer.
Explanation
Boiling point elevation depends on the total concentration of dissolved particles. Calcium chloride dissociates into three ions per formula unit (i ≈ 3), exceeding the two particles from NaCl or KCl and the single particle from glucose.
Question 26Survey of the Natural Sciences
Which organelle is the primary site of intracellular digestion of macromolecules and worn-out organelles?
- Peroxisome
- Lysosome Correct answer.
- Ribosome
- Golgi apparatus
Explanation
Lysosomes contain hydrolytic enzymes that operate at acidic pH and degrade macromolecules and damaged organelles delivered by vesicle fusion. Ribosomes synthesize proteins, the Golgi modifies and sorts proteins, and peroxisomes carry out oxidative reactions such as fatty acid breakdown and hydrogen peroxide metabolism.
Question 27Survey of the Natural Sciences
In a dihybrid cross between two individuals heterozygous at both loci (AaBb × AaBb), assuming independent assortment and complete dominance, what phenotypic ratio is expected?
- 3:1
- 1:2:1
- 9:3:3:1 Correct answer.
- 1:1:1:1
Explanation
Each locus in an Aa × Aa cross gives a 3:1 phenotypic ratio, and independent assortment allows the two loci to be multiplied, yielding 9:3:3:1. The 1:1:1:1 ratio arises from a testcross with a double heterozygote, and 3:1 describes a monohybrid cross.
Question 28Survey of the Natural Sciences
Which of the following elements has the highest electronegativity?
- Fluorine Correct answer.
- Sodium
- Chlorine
- Oxygen
Explanation
Electronegativity increases across a period and decreases down a group, placing fluorine at the upper-right corner of the periodic table with the highest value. Oxygen is also highly electronegative but lies to the left of fluorine in the same period.
Question 29Survey of the Natural Sciences
Which functional group characterizes a carboxylic acid?
- Carboxyl (–COOH) Correct answer.
- Amino (–NH2)
- Aldehyde (–CHO)
- Hydroxyl (–OH)
Explanation
A carboxylic acid contains a carbonyl carbon bonded directly to a hydroxyl group, giving the –COOH group and its acidic proton. Hydroxyl groups alone define alcohols, aldehydes end in –CHO, and amino groups define amines.
Question 30Survey of the Natural Sciences
In oxygenic photosynthesis, the oxidation of water to molecular oxygen occurs at which photosystem?
- Cytochrome b6f
- Photosystem II Correct answer.
- Photosystem I
- ATP synthase
Explanation
The oxygen-evolving complex associated with photosystem II splits water to replace the electrons lost from its reaction center, releasing O2 and protons. Photosystem I re-energizes electrons for NADPH formation, while the cytochrome b6f complex and ATP synthase handle electron transport and proton-driven ATP synthesis.
Question 31Survey of the Natural Sciences
A fixed amount of ideal gas is held at constant temperature. If its volume is reduced to one-half of its initial value, the pressure will:
- It doubles Correct answer.
- It halves
- It stays the same
- It quadruples
Explanation
At constant temperature and amount, Boyle's law states that pressure and volume are inversely proportional (PV = constant). Halving the volume therefore doubles the pressure.
Question 32Survey of the Natural Sciences
The central nervous system develops from which embryonic germ layer?
- Ectoderm Correct answer.
- Trophoblast
- Mesoderm
- Endoderm
Explanation
The ectoderm gives rise to the neural tube, which forms the brain and spinal cord, as well as the epidermis. The mesoderm forms muscle, bone, and connective tissues, while the endoderm lines internal organs.
Question 33Survey of the Natural Sciences
Which of the following processes is endothermic?
- Combustion of methane
- Condensation of steam
- Melting of ice Correct answer.
- Freezing of water
Explanation
Endothermic processes absorb heat because the products have higher enthalpy than the reactants. Melting requires energy to disrupt the hydrogen-bonded lattice of ice, whereas condensation, freezing, and combustion release heat.
Question 34Survey of the Natural Sciences
During DNA replication, which enzyme seals the nicks between Okazaki fragments by forming phosphodiester bonds?
- Helicase
- DNA polymerase III
- DNA ligase Correct answer.
- Primase
Explanation
DNA ligase joins the 3′-hydroxyl of one fragment to the 5′-phosphate of the next, restoring the phosphodiester backbone. DNA polymerase III extends primers, helicase unwinds the duplex, and primase synthesizes RNA primers.
Question 35Survey of the Natural Sciences
A first-order reaction has a half-life of 30 minutes. What fraction of the original reactant remains after 90 minutes?
- 1/8 Correct answer.
- 1/2
- 1/4
- 1/16
Explanation
Ninety minutes corresponds to three half-lives (90 ÷ 30 = 3). Each half-life halves the remaining amount, so the fraction remaining is (1/2)^3 = 1/8.
Question 36Survey of the Natural Sciences
For the exothermic equilibrium N2(g) + 3 H2(g) ⇌ 2 NH3(g), which change increases the equilibrium yield of NH3?
- Removing nitrogen gas
- Adding a catalyst
- Increasing the temperature
- Compressing the gas mixture Correct answer.
Explanation
Compression raises the partial pressures and shifts the equilibrium toward the side with fewer moles of gas, which is the product side (2 mol versus 4 mol). Because the forward reaction is exothermic, raising temperature shifts the equilibrium toward reactants.
Question 37Survey of the Natural Sciences
What is the conjugate base of HSO4−?
- H2SO4
- OH−
- H3O+
- SO4^2− Correct answer.
Explanation
A conjugate base forms when an acid donates a proton. Removing H+ from HSO4− leaves SO4^2−, which can accept a proton to regenerate HSO4−.
Question 38Survey of the Natural Sciences
When a nucleus undergoes alpha emission, the mass number of the daughter nucleus is:
- Increased by 4
- Decreased by 4 Correct answer.
- Decreased by 2
- Unchanged
Explanation
An alpha particle is a helium-4 nucleus, containing two protons and two neutrons. Its emission therefore lowers the mass number by 4 and the atomic number by 2.
Question 39Survey of the Natural Sciences
Reaction of a Grignard reagent (RMgBr) with a ketone, followed by aqueous acid workup, produces which type of alcohol?
- Carboxylic acid
- Primary alcohol
- Tertiary alcohol Correct answer.
- Secondary alcohol
Explanation
The Grignard carbon attacks the ketone carbonyl, converting the carbonyl carbon into an alcohol carbon that already bears two alkyl groups. Adding the new R group makes three carbon substituents, so the product is a tertiary alcohol.
Question 40Survey of the Natural Sciences
In the human nephron, most filtered glucose is reabsorbed in which segment?
- Collecting duct
- Distal tubule
- Proximal tubule Correct answer.
- Descending limb
Explanation
The proximal tubule contains sodium-glucose cotransporters that reclaim nearly all filtered glucose under normal conditions. The descending limb is water-permeable but reabsorbs little glucose, and the distal nephron handles fine-tuning of ions and water.
Question 41Survey of the Natural Sciences
Which process allows a bacterium to take up naked DNA directly from its surroundings?
- Binary fission
- Transduction
- Conjugation
- Transformation Correct answer.
Explanation
Transformation is the uptake of free DNA from the environment, which can then be incorporated into the bacterial genome. Conjugation requires cell-to-cell contact, transduction is phage-mediated, and binary fission is reproduction.
Question 42Survey of the Natural Sciences
Random fluctuations in allele frequencies from generation to generation in a very small population are best described as:
- Natural selection
- Stabilizing selection
- Gene flow
- Genetic drift Correct answer.
Explanation
Genetic drift is the stochastic change in allele frequencies caused by sampling effects, and it is strongest in small populations. Natural selection and stabilizing selection are nonrandom processes based on fitness, while gene flow involves migration.
Question 43Survey of the Natural Sciences
Which of the following species is aromatic?
- Cyclooctatetraene (8 π electrons)
- Cyclobutadiene (4 π electrons)
- Cyclopentadienyl anion Correct answer.
- Cyclopentyl cation (4 π electrons)
Explanation
The cyclopentadienyl anion is planar, fully conjugated, and contains 6 π electrons, satisfying the Hückel 4n + 2 rule with n = 1. Cyclobutadiene has 4 π electrons and is antiaromatic, cyclooctatetraene avoids planarity and is nonaromatic, and the cyclopentyl cation is not fully conjugated.
Question 44Survey of the Natural Sciences
Which reagent is commonly used to distinguish an aldehyde from a ketone?
- Sodium metal
- Bromine water
- Lucas reagent (ZnCl2/HCl)
- Tollens' reagent Correct answer.
Explanation
Tollens' reagent contains Ag(NH3)2+, which oxidizes aldehydes to carboxylates while depositing a silver mirror; ketones lack the required aldehydic hydrogen and do not react. Bromine water tests unsaturation, Lucas reagent tests alcohol substitution, and sodium metal reacts with acidic protons such as alcohols.
Question 45Survey of the Natural Sciences
Which of the following molecules has a net dipole moment of zero?
- NH3
- H2O
- CO2 Correct answer.
- HCl
Explanation
CO2 is linear and its two polar C=O bonds point in opposite directions, so the bond dipoles cancel exactly. Water is bent, ammonia is trigonal pyramidal, and HCl is a polar diatomic molecule, so each has a nonzero net dipole.
Question 46Survey of the Natural Sciences
A woman with blood type AB and a man with blood type O have a child. Which ABO blood types are possible in their offspring?
- All four types
- Types A and B only Correct answer.
- Types AB and O only
- Type A only
Explanation
The mother has genotype IAIB and the father has genotype ii. Each child inherits one allele from each parent, yielding either IAi (type A) or IBi (type B); type AB and type O are impossible.
Question 47Survey of the Natural Sciences
Which step in glycolysis generates ATP by substrate-level phosphorylation?
- Fructose-6-phosphate → fructose-1,6-bisphosphate
- 1,3-Bisphosphoglycerate → 3-phosphoglycerate Correct answer.
- Dihydroxyacetone phosphate → glyceraldehyde-3-phosphate
- Glucose → glucose-6-phosphate
Explanation
In the phosphoglycerate kinase reaction, the high-energy acyl phosphate of 1,3-bisphosphoglycerate transfers a phosphate to ADP, forming ATP directly. The first two listed steps consume ATP, and the triose phosphate isomerase step is only an isomerization.
Question 48Survey of the Natural Sciences
Which of the following compounds is the most acidic?
- p-Methoxyphenol
- p-Nitrophenol Correct answer.
- Phenol
- Cyclohexanol
Explanation
The nitro group is strongly electron-withdrawing by both induction and resonance, stabilizing the phenoxide conjugate base and lowering the pKa relative to phenol. A methoxy group is electron-donating and destabilizes the phenoxide, while cyclohexanol lacks resonance stabilization entirely.
Question 49Survey of the Natural Sciences
A 0.10 M solution of a weak monoprotic acid has a pH of 3.00 at 25 °C. What is the approximate Ka of the acid?
- 1 × 10−3
- 1 × 10−4
- 1 × 10−6
- 1 × 10−5 Correct answer.
Explanation
A pH of 3.00 means [H+] = 1 × 10−3 M. For HA ⇌ H+ + A−, Ka ≈ [H+]^2/[HA] = (1 × 10−3)^2/0.10 = 1 × 10−5, since the amount dissociated is small relative to 0.10 M.
Question 50Survey of the Natural Sciences
Which compound gives a positive iodoform test?
- Cyclohexanone
- Benzaldehyde
- 3-Pentanone
- Acetone Correct answer.
Explanation
The iodoform test is positive for methyl ketones and for compounds oxidized to acetaldehyde, because the required CH3CO– unit reacts with iodine and base to form CHI3. Acetone is the only methyl ketone among the choices.