Maharashtra HSC Medical Lab Technology (Vocational) study plan

Map the chain of infection to laboratory practice by naming three engineering controls, three PPE items and three work-practice controls, and stating which hazard each blocks.

Write out the step-by-step sequence for hand hygiene, glove doffing and a 10% bleach spill clean-up, in correct order, without referring to notes.

Classify given QC scenarios under Westgard rules (1-2s, 1-3s, 2-2s, R-4s, 10x) and state the corrective action for each.

Sequence first-aid response for chemical eye splash, needle-stick injury and fume inhalation, giving timings and the exact wash/flush duration for each.

Name the eleven organ systems and state one measurable function of each that a laboratory test can reflect.

Trace the path of blood through the heart chambers and valves and relate each chamber to the vessel it feeds.

Segment the nephron and state what is filtered, reabsorbed or secreted at each segment.

Describe the life cycle of an erythrocyte, the structure of haemoglobin and the site of production of each formed element.

Distinguish acute from chronic inflammation and benign from malignant neoplasia using cellular features rather than definitions alone.

Work through five short pathology scenarios (oedema, thrombosis, embolism, infarction, anaemia) and state the underlying mechanism for each.

Convert concentrations between mg/dL, mmol/L, g/L and mEq/L for glucose, urea, creatinine, cholesterol and bilirubin using the correct factors.

Explain the reaction principle, reagent role and interference for glucose (glucose oxidase and hexokinase), urea (urease), creatinine (Jaffe) and uric acid methods.

Classify jaundice as pre-hepatic, hepatic or post-hepatic by interpreting a set of given bilirubin, ALT, AST and ALP values.

Build a lipid profile and cardiac marker interpretation table linking each analyte to the condition it flags.

Identify causes of falsely high or falsely low results for at least eight common biochemistry analytes.

Apply the Beer-Lambert law to calculate unknown concentrations from absorbance and to explain linearity limits.

Describe calibration, daily checks and fault-finding for a spectrophotometer, colorimeter, analytical balance, water bath and micropipette.

Prepare calculation sets for molar, normal, percent and dilution-series solutions and spot errors in three faulty preparations.

Explain the principle and use of a pH meter, centrifuge, refrigerator and automated biochemistry analyser, including one common failure of each.

Outline the calibration and quality-control documentation required before a biochemistry run is reported.

Describe the manual method, reference range and three sources of error for haemoglobin estimation, packed cell volume, ESR and total leucocyte count.

Classify anaemias as microcytic, normocytic or macrocytic by calculating MCV, MCH and MCHC from given red cell indices.

Explain the coagulation cascade, name the pathway tested by PT/INR and by aPTT, and state the sample, anticoagulant and storage limit for each.

Perform forward and reverse ABO grouping and Rh typing on paper for six given cell/serum patterns and flag discrepancies.

Build a major crossmatch compatibility grid and state the action required for an incompatible unit.

List the preparation, storage temperature, shelf life and one quality check for whole blood, packed cells, platelet concentrate, fresh frozen plasma and cryoprecipitate.

Tabulate ten transfusion reactions with cause, presenting sign, immediate action and preventive step.

Compare autoclave, hot air oven, membrane filtration and chemical disinfection by parameters, monitoring method and material suitability.

Prepare a scheme classifying culture media by consistency, oxygen requirement and purpose, citing one example of each.

Write the Gram stain procedure step by step with timings, and interpret given microscopy descriptions as Gram-positive or Gram-negative with morphology.

Explain the bacterial growth curve and the use of catalase, coagulase, oxidase and IMViC tests in identification.

Match ten common pathogens to their typical specimen, culture medium and confirmatory test.

Describe Ziehl-Neelsen staining, culture and drug-susceptibility reporting for tuberculosis, including biosafety requirements.

Identify malaria parasite stages on a described thick and thin film, and outline stool examination for ova and cysts.

Interpret a full urinalysis (physical, chemical, microscopic) and a CSF or pleural fluid report for three given cases.

Sequence tissue processing from fixation through dehydration, clearing, embedding, sectioning and H&E staining, naming the reagent and purpose at each step.

Compare exfoliative and aspiration cytology, and describe the Pap smear sampling, fixation and reporting categories.

Distinguish ELISA, agglutination, precipitation, immunochromatography and Western blot by principle, use and one limitation each.

Interpret serological titres and explain prozone and postzone phenomena with a corrective step.

Sit a timed 60-question mixed mock covering all nine domains and review every error.