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Double fertilization
In angiosperms one male gamete fuses with the egg to form a diploid zygote, while the second fuses with two polar nuclei to form triploid endosperm.
Porogamy
Entry of the pollen tube into the ovule through the micropyle; the most common route of fertilization in angiosperms.
Spermatogenesis output
One primary spermatocyte completes meiosis to produce four haploid spermatids, which mature into four functional sperm cells.
Site of fertilization
In humans, fusion of sperm and ovum normally occurs in the ampullary-isthmic junction of the fallopian tube, not in the uterus.
Monohybrid genotypic ratio
In the F2 generation of a monohybrid cross the genotypes are in the ratio 1 homozygous dominant : 2 heterozygous : 1 homozygous recessive.
Dihybrid F2 ratio
When two independently assorting gene pairs are crossed, the F2 phenotypic ratio is 9:3:3:1, assuming no linkage or lethality.
Test cross
Crossing an individual of unknown genotype with a homozygous recessive partner; a 1:1 ratio reveals a heterozygote.
Semiconservative replication
Each daughter DNA molecule retains one parental strand and one newly synthesized strand, as shown by the Meselson-Stahl experiment.
Chargaff's equivalence rule
In double-stranded DNA the amount of adenine equals thymine and guanine equals cytosine, so purines equal pyrimidines overall.
Lac operon
An inducible operon switched on when lactose binds the repressor, allowing RNA polymerase to transcribe lacZ, lacY and lacA.
Hardy-Weinberg conditions
Allele frequencies stay constant only with no mutation, no migration, random mating, no selection and a very large population.
Hardy-Weinberg equation
p + q = 1 for allele frequencies, and p squared plus 2pq plus q squared = 1 for genotype frequencies in a stable population.
HIV replication
HIV is a retrovirus; reverse transcriptase converts its RNA genome into DNA, which integrates into the host genome and infects helper T cells.
Malaria transmission
Plasmodium species are transmitted by the bite of an infected female Anopheles mosquito, which injects sporozoites into the human blood.
Restriction endonuclease
A bacterial enzyme that cuts DNA at a specific palindromic sequence, producing sticky or blunt ends for recombinant DNA work.
EcoRI recognition site
The enzyme recognizes the palindromic hexanucleotide GAATTC and cuts between G and A, generating complementary sticky ends.
Ti plasmid
A tumour-inducing plasmid from Agrobacterium tumefaciens used as a natural vector to deliver genes into plant cells.
PCR cycle steps
Repeated cycles of denaturation, primer annealing and extension by Taq polymerase double the target DNA each round.
Ten percent law
Only about ten percent of energy at one trophic level is transferred to the next; the rest is lost as heat and respiration.
Net primary productivity
NPP equals gross primary productivity minus the energy lost through respiration by producers; it is the energy available to consumers.
Logistic growth equation
dN/dt = rN(K-N)/K describes population growth slowing as N approaches carrying capacity K, producing a sigmoid curve.
Exponential growth equation
dN/dt = rN describes unlimited growth with constant intrinsic rate r, giving a J-shaped curve when resources are abundant.
Loop of Henle counter-current
The descending and ascending limbs maintain a medullary osmotic gradient, allowing concentrated urine to be formed under ADH.
Sinoatrial node
A patch of pacemaker cells in the right atrium that spontaneously depolarizes and sets the rhythm of the heartbeat.
Bohr effect
A fall in pH or rise in carbon dioxide reduces hemoglobin's affinity for oxygen, shifting the dissociation curve to the right.
Transpiration pull
Water is pulled up the xylem by evaporation from leaves, creating tension transmitted through continuous water columns.
Photosystem II role
PSII absorbs light and splits water, releasing oxygen and supplying electrons to the photosynthetic electron transport chain.
Calvin cycle
Light-independent reactions in which RuBisCO fixes carbon dioxide onto RuBP, producing sugars through reduction and regeneration steps.
Glycolysis net yield
One glucose yields two pyruvate, a net two ATP and two NADH during glycolysis in the cytoplasm, independent of oxygen.
Krebs cycle location
In eukaryotes the citric acid cycle occurs in the mitochondrial matrix and yields NADH, FADH2, ATP and carbon dioxide.
Mendel's law of segregation
The two alleles of a gene separate during gamete formation, so each gamete carries only one allele of each pair.
Sex-linked inheritance
Genes on the X chromosome show different inheritance in males and females; affected fathers cannot pass X-linked traits to sons.