NHA CPT study materials
NHA CPT study resources

NHA CPT key terms

NHA CPT · Get comfortable with the vocabulary.

Standard Precautions

A set of infection-control practices applied to every patient regardless of diagnosis, including hand hygiene, gloves, gowns, masks, and eye protection when contact with blood or body fluids is likely.

Related: Personal Protective Equipment, Hand Hygiene, Exposure Control Plan
Personal Protective Equipment (PPE)

Barrier items such as gloves, gowns, masks, respirators, and eye protection worn to prevent skin, clothing, and mucous membranes from contacting infectious material or hazardous chemicals.

Related: Standard Precautions, Doffing Sequence
Hand Hygiene

Cleaning hands with soap and water or an alcohol-based rub at defined moments, such as before and after patient contact, after glove removal, and after touching contaminated surfaces or specimens.

Related: Standard Precautions, Personal Protective Equipment
Doffing Sequence

The ordered removal of contaminated protective equipment so that clean areas are not touched by soiled surfaces, generally gloves first, then face protection, then gown, with hand hygiene afterward.

Related: Personal Protective Equipment, Hand Hygiene
Exposure Control Plan

A written employer program describing how bloodborne pathogen risks are identified and controlled, including engineering controls, work practice controls, vaccination offers, and post-exposure procedures.

Related: Standard Precautions, Post-Exposure Prophylaxis
Post-Exposure Prophylaxis

Medical evaluation and preventive treatment offered after a needlestick, splash, or other occupational exposure, including source testing and counseling for bloodborne pathogens such as hepatitis B and HIV.

Related: Exposure Control Plan, Sharps Injury Log
Sharps Injury Log

A confidential record of percutaneous injuries from needles, lancets, or broken glass, documenting the device involved, the circumstances, and the exposure route for infection-control review and prevention.

Related: Post-Exposure Prophylaxis, Sharps Container
Sharps Container

A puncture-resistant, closable, labeled receptacle used to dispose of needles, lancets, and other sharp items immediately after use, replaced when about three-quarters full.

Related: Sharps Injury Log, Needle Safety Device
Needle Safety Device

An engineered sharp with a shield, retracting needle, or similar feature that reduces needlestick risk after collection, required in most settings under bloodborne pathogen regulations.

Related: Sharps Container, Sharps Injury Log
Chain of Custody

A documented, unbroken record of who collected, handled, stored, and transported a specimen, used for forensic, legal, or employment testing where identity and integrity must be provable.

Related: Specimen Rejection, Forensic Specimen
Specimen Rejection Criteria

Predefined conditions that make a sample unacceptable for testing, such as missing identification, inadequate volume, wrong tube, hemolysis, or delayed delivery beyond stability limits.

Related: Chain of Custody, Hemolysis
Biohazard Labeling

Marking containers and transport bags with the required biohazard symbol and text so handlers recognize infectious material and follow appropriate precautions throughout transport.

Related: Secondary Container, Standard Precautions
Patient Identification Verification

Confirming identity using at least two independent identifiers, such as full name and date of birth, plus matching the requisition before any collection or procedure.

Related: Requisition, Informed Consent
Informed Consent

A patient's voluntary agreement to a procedure after being told its purpose, what to expect, risks, and alternatives, documented according to facility policy before the collection begins.

Related: Patient Identification Verification, Requisition
Requisition

The order authorizing a test, listing patient identifiers, the requested tests, collection date and time, ordering provider, and any special handling instructions the collector must follow.

Related: Patient Identification Verification, Test Menu
Fasting Specimen

A sample drawn after a required period without food or calorie-containing drink, commonly eight to twelve hours, so glucose, lipids, and similar analytes reflect baseline values.

Related: Timed Specimen, Basal State
Basal State

The resting condition of a patient after an overnight fast and without strenuous activity, used as a reference condition when collecting specimens for certain baseline laboratory measurements.

Related: Fasting Specimen, Timed Specimen
Timed Specimen

A sample collected at a specified clock time or interval after an event, such as a drug dose or meal, because the analyte's concentration changes predictably with time.

Related: Fasting Specimen, Therapeutic Drug Monitoring
Therapeutic Drug Monitoring

Serial measurement of a drug's blood level to keep it within a therapeutic range, requiring collections timed relative to dosing and documented precisely on the requisition.

Related: Timed Specimen, Trough Level
Trough Level

The lowest concentration of a drug in blood, typically drawn just before the next dose, used to guide dosing and avoid toxicity for drugs with narrow therapeutic windows.

Related: Therapeutic Drug Monitoring, Peak Level
Peak Level

The highest concentration of a drug in blood after administration, collected at a defined interval after the dose, used with trough values to assess dosing adequacy.

Related: Trough Level, Therapeutic Drug Monitoring
Patient Preparation Instructions

Directions given before collection about fasting, medication holds, activity restrictions, or timing, so the specimen reflects the intended physiologic state and avoids avoidable recollections.

Related: Fasting Specimen, Basal State
Venipuncture Site Selection

Choosing a suitable vein by palpation, usually in the antecubital area, avoiding areas with scarring, hematoma, intravenous lines, dialysis fistulas, or recent venipuncture.

Related: Order of Draw, Tourniquet Application
Tourniquet Application

Tying a constricting band above the puncture site to distend veins, left in place no longer than about one minute because prolonged stasis alters analyte concentrations.

Related: Venipuncture Site Selection, Hemoconcentration
Order of Draw

The mandated sequence for filling blood tubes during a single puncture, arranged so additive carryover from one tube does not contaminate the next and results stay accurate.

Related: Additive Carryover, Blood Culture Collection
Additive Carryover

Transfer of an additive such as EDTA or heparin from one tube into the next through the needle, which can distort coagulation or chemistry results if the draw order is wrong.

Related: Order of Draw, EDTA Tube
Blood Culture Collection

Aseptic collection of blood sets, usually two sets from separate sites, into dedicated culture bottles, drawn before antimicrobial therapy and with careful skin antisepsis.

Related: Order of Draw, Antisepsis
Antisepsis

Applying an antimicrobial agent to skin or mucous membranes to reduce resident and transient organisms before a puncture, allowing the required contact time to dry.

Related: Blood Culture Collection, Venipuncture Site Selection
Capillary Puncture

Collection of a small blood volume by pricking a fingertip or heel, used when venous access is impractical, with the first drop sometimes wiped away per device instructions.

Related: Capillary Order of Draw, Lancet Depth
Capillary Order of Draw

The sequence for filling microcollection tubes from a skin puncture, generally starting with tubes for blood gases or EDTA and ending with tubes containing other additives.

Related: Capillary Puncture, Additive Carryover
Lancet Depth

The penetration setting chosen for a skin puncture device, selected by patient age and site so the incision reaches capillary beds without striking bone or causing unnecessary injury.

Related: Capillary Puncture, Heel Puncture Site
Heel Puncture Site

The lateral or medial plantar surface of an infant's heel used for capillary blood, avoiding the central area and posterior curve to prevent bone injury and nerve damage.

Related: Lancet Depth, Capillary Puncture
Hemoconcentration

A rise in concentration of cells and large molecules in the sampled blood caused by prolonged tourniquet time, dehydration, or stasis, which can falsely elevate some analyte results.

Related: Tourniquet Application, Hemolysis
Hemolysis

Rupture of red blood cells that releases hemoglobin into plasma or serum, often from rough handling, small needles, or forceful syringe draws, and which interferes with many assays.

Related: Specimen Rejection Criteria, Serum Separator Tube
Serum Separator Tube

A tube containing a clot activator and a gel barrier that, after centrifugation, separates serum from cells so the serum can be analyzed or stored without cell contact.

Related: Centrifugation, EDTA Tube
EDTA Tube

A lavender-stoppered tube containing ethylenediaminetetraacetic acid, which binds calcium to prevent clotting, used for hematology tests such as complete blood counts.

Related: Additive Carryover, Order of Draw
Centrifugation

Spinning a specimen at a specified speed and duration to separate components by density, with balance, time, and speed controlled to preserve sample integrity.

Related: Serum Separator Tube, Hemolysis
Specimen Stability

The period and conditions under which a sample retains acceptable analyte values, governing how quickly it must be processed, refrigerated, frozen, or protected from light.

Related: Transport Temperature, Specimen Rejection Criteria
Transport Temperature

The required thermal condition for moving a specimen, such as ambient, refrigerated, frozen, or protected from light, chosen to preserve the analytes being measured.

Related: Specimen Stability, Cold Chain
Cold Chain

An uninterrupted series of refrigerated or frozen handling steps from collection through testing, maintained with validated coolers, monitoring, and documented temperature records.

Related: Transport Temperature, Specimen Stability
Secondary Container

A leak-resistant outer packaging layer that holds primary specimen containers with absorbent material, protecting carriers and the environment if a primary container breaks.

Related: Biohazard Labeling, Transport Temperature