SANE-A study materials
SANE-A study resources

SANE-A cheat sheet

SANE-A · Keep the key ideas within reach.

Quick reference

SANE Role, Scope of Practice, and Professional Issues

  • SANE-A certification is issued by the American Nurses Credentialing Center (ANCC) and covers adolescent and adult patients; SANE-P covers prepubertal children.

  • Scope of practice is set by the state Nurse Practice Act plus institutional policy and protocols; a SANE cannot independently order or prescribe outside that authority.

  • Informed consent for the forensic examination must be voluntary and revocable; patients may accept medical care and decline any or all evidence collection.

  • The SANE role combines medical-forensic history, physical assessment, injury identification, evidence collection, documentation, prophylaxis, referral, and testimony.

  • A fact witness testifies to direct observations; an expert witness may offer opinions grounded in specialized education, training, and experience.

  • Chain of custody must be documented at every transfer, including date, time, releasing and receiving party, item description, and purpose of transfer.

  • HIPAA permits disclosure of protected health information for reporting abuse, neglect, or violent injury as required or permitted by law.

  • Mandatory reporting obligations are defined by state statute and override patient requests for confidentiality when the statutory trigger is met.

  • Certification renewal requires continued clinical practice hours and continuing education; a lapsed credential affects eligibility to practice as a certified SANE.

Quick reference

Anatomy, Physiology, and Patient Assessment (Adult and Adolescent)

  • Structures requiring systematic inspection: mons pubis, labia majora and minora, clitoris, urethral meatus, hymen, fossa navicularis, posterior fourchette, perineum, and anus.

  • Tanner stages 1 through 5 describe breast and pubic hair development; estrogenized hymenal tissue is thick, redundant, and moist, whereas prepubertal tissue is thin and delicate.

  • Hymenal morphology varies normally: annular, crescentic, redundant, septate, cribriform, and microperforate configurations are all described variants.

  • The posterior fourchette, fossa navicularis, and labia minora are the most frequently injured genital sites following penile penetration.

  • Examination positions include lithotomy (supine, legs abducted), lateral recumbent, and prone knee-chest, which improves visualization of the anus and posterior vagina.

  • Magnification with a colposcope and bright, angled lighting improves detection of mucosal tears not visible on gross inspection.

  • The anal examination inspects perianal skin, anal folds, and sphincter tone and requires explicit separate consent.

  • Baseline vital signs, pain score, and orthostatic measurements guide triage for hypovolemia and hemodynamic instability before the forensic examination proceeds.

  • Oral assessment includes lips, frenulum, buccal mucosa, palate, and posterior pharynx for petechiae, tears, or bruising.

Quick reference

Forensic Evidence Identification, Collection, and Preservation

  • DNA-bearing body swabs are generally collected up to about 120 hours post-assault; clothing, bedding, and other items may be collected beyond that window.

  • Each swab is air-dried, placed in a paper envelope, sealed with tamper-evident tape, and labeled with patient identifiers, collector name, site, date, and time.

  • Plastic bags are avoided for biological evidence because trapped moisture promotes bacterial growth and DNA degradation.

  • A known reference sample (buccal swab or blood) from the patient is required to distinguish patient DNA from foreign DNA.

  • Toxicology specimens (blood and urine) are refrigerated if not delivered promptly; urine has a longer detection window for most substances.

  • Clothing is collected in paper bags; wet or heavily stained items are air-dried before packaging.

  • Every transfer of evidence is logged so the chain of custody remains unbroken from collection through laboratory analysis and return.

  • Evidence the patient declines cannot be collected; the refusal is documented and care continues.

  • Kits are stored per jurisdictional rules (often refrigerated or controlled room temperature) and held for the statutory retention period.

Quick reference

Trauma-Informed Examination and Injury Identification (Including Strangulation and Medical Stability)

  • Non-fatal strangulation leaves no visible external marks in roughly half of cases; absence of visible injury does not exclude strangulation.

  • Documented signs include facial and conjunctival petechiae, subconjunctival hemorrhage, ligature marks, neck swelling, voice change, cough, dysphagia, and odynophagia.

  • Loss of consciousness, incontinence, visual changes, and respiratory symptoms are high-risk indicators requiring emergency evaluation.

  • Airway edema may worsen over 24 to 72 hours; discharge instructions must list return precautions for breathing difficulty, voice change, or neck swelling.

  • Stabilization of life-threatening injury takes priority over evidence collection; collection resumes once the patient is medically stable.

  • Trauma-informed sequence: explain before each step, obtain permission, allow a support person, offer breaks, minimize repeat retelling, and let the patient set the order.

  • Injuries are documented with type, size in centimeters, color, shape, anatomic location, and a body diagram.

  • Photographs are taken with consent, with and without a scale, and recorded in a log noting time, photographer, and body location.

  • Many states classify strangulation as a felony independent of visible injury, and standardized strangulation documentation forms are recommended.

Quick reference

Prophylaxis, Treatment, and Follow-Up Medical Management (STI, Pregnancy, HIV)

  • HIV nPEP: initiate within 72 hours of exposure, ideally within 2 hours; continue for 28 days; preferred adult regimen is tenofovir DF/emtricitabine plus dolutegravir.

  • STI prophylaxis: ceftriaxone 500 mg IM once (1 g if weight 150 kg or greater), doxycycline 100 mg PO twice daily for 7 days, metronidazole 2 g PO once.

  • Pregnancy alternative: azithromycin 1 g PO once replaces doxycycline; metronidazole timing is adjusted in some first-trimester protocols.

  • Hepatitis B: unvaccinated patients receive hepatitis B immune globulin plus the first vaccine dose, with the series completed per schedule.

  • Emergency contraception: copper IUD up to 5 days (most effective), ulipristal acetate 30 mg up to 120 hours, levonorgestrel 1.5 mg up to 72 hours with reduced efficacy to 120 hours.

  • Baseline testing includes pregnancy, HIV, syphilis, hepatitis B and C, and nucleic acid amplification tests for gonorrhea and chlamydia.

  • Repeat HIV testing is recommended at approximately 6 weeks, 3 months, and 6 months after exposure; syphilis serology is repeated at later intervals.

  • HPV vaccination is offered according to age-based recommendations during the follow-up visit.

Quick reference

Psychosocial, Cultural, and Behavioral Health Considerations

  • Rape trauma syndrome: an acute phase of disorganization (fear, shock, somatic complaints) followed by a long-term reorganization phase (phobias, anxiety, depression, altered relationships).

  • Tonic immobility, an involuntary freeze response during assault, is a documented survival reaction and is never evidence of consent.

  • Memory during highly stressful events is fragmented; inconsistent recall of peripheral detail is expected and does not indicate fabrication.

  • Use trained interpreters rather than family members or children; an accompanying person may be the assailant.

  • Screen for suicidal ideation, depression, PTSD symptoms, and substance use, and provide crisis resources with a warm handoff.

  • Populations facing added barriers include male survivors, LGBTQ+ patients, people with disabilities, incarcerated persons, immigrants, and trafficking victims.

  • Cultural factors shape disclosure, eye contact, and willingness to undress; ask about examiner gender preference and use of a chaperone.

  • Absence of visible distress, flat affect, or calm cooperation is a common trauma response and does not suggest the assault did not occur.

Quick reference

Legal, Ethical, and Judicial Proceedings (Including Courtroom Testimony)

  • A fact witness testifies only to personal observations; an expert witness may give opinion testimony based on specialized knowledge after qualification by the court.

  • Subpoenas for records must be validated through hospital legal counsel or risk management before any protected health information is released.

  • Rape shield statutes generally bar evidence of the complainant's prior sexual conduct or reputation except in narrow statutorily defined circumstances.

  • Consent is the ethical and legal foundation of the examination; a patient may withdraw consent at any time and documentation must reflect the scope consented to.

  • Mandatory reporting requirements for child abuse, elder abuse, and specified injuries are statutory and override confidentiality.

  • A break in chain of custody can render evidence inadmissible; documentation must show continuous accountability for each item.

  • Testimony rules: answer only the question asked, avoid speculation, use plain language, and state uncertainty rather than guessing.

  • Adolescent consent rules differ by state; emancipation status and minor-consent statutes determine who may authorize examination and treatment.

Quick reference

Documentation, Quality Improvement, and Evidence-Based Practice

  • Documentation must be objective, timed, and measurable: record size in centimeters, shape, color, and anatomic location rather than conclusory labels.

  • Patient statements are recorded as direct quotations attributed to the patient and kept distinct from examiner observations.

  • Body diagrams and a photography log (time, photographer, location, scale) support later authentication of images in court.

  • The IAFN Standards of Practice and CDC treatment guidelines are the principal evidence-based references for forensic nursing care.

  • Quality indicators include time from report to examination, kit completion rate, documentation completeness, chain-of-custody accuracy, and follow-up completion.

  • Case review and peer review identify documentation gaps that drive protocol revision and staff education.

  • Evidence retention periods are set by state statute and institutional policy and must be confirmed before any kit destruction.

  • Appraisal of new research for practice change includes assessment of study design, sample size, and applicability to the local patient population.