ABSA CBSP study materials
ABSA CBSP study resources

ABSA CBSP key terms

ABSA CBSP · Get comfortable with the vocabulary.

Risk assessment

A structured process of identifying hazardous agents and activities, then estimating the likelihood and consequences of exposure so that controls can be chosen and justified.

Related: Risk group, Mitigation
Risk group

A classification category that groups biological agents by the severity of disease they cause and the availability of effective treatment or prophylaxis, independent of the laboratory controls used.

Related: Biosafety level, Risk assessment
Biosafety level

A defined set of facility design features, equipment, and work practices assigned to laboratory work according to the assessed risk of the agents handled, commonly numbered one through four.

Related: Risk group, Primary containment, Secondary containment
Mitigation

Any action, device, or administrative measure taken to reduce the probability or severity of a biological exposure or release after a risk has been identified and evaluated.

Related: Risk assessment, Hierarchy of controls
Hierarchy of controls

A ranked framework that prefers eliminating or engineering out a hazard before relying on administrative rules or personal protective equipment, because higher-ranked controls protect more people more reliably.

Related: Mitigation, Engineering control
Primary containment

The first barrier that keeps infectious material away from people and the room, typically provided by a biological safety cabinet, sealed centrifuge cup, or other equipment enclosing the hazard.

Related: Biological safety cabinet, Secondary containment
Secondary containment

The room, suite, or building-level barriers and ventilation features that protect people outside the immediate work area and the surrounding environment if primary containment fails.

Related: Primary containment, Directional airflow
Biological safety cabinet

A ventilated enclosed work station that uses inward airflow and high-efficiency filtration to protect the operator, the work surface, or both from aerosols generated during handling of biological material.

Related: Primary containment, HEPA filter, Certification
HEPA filter

A high-efficiency particulate air filter that removes at least 99.97 percent of particles of 0.3 micrometer diameter, used to clean exhaust or supply air in containment systems.

Related: Biological safety cabinet, Directional airflow
Directional airflow

Deliberate movement of air from cleaner corridors into dirtier work areas and out through filtered exhaust, so that airborne contamination travels away from people and clean spaces.

Related: Secondary containment, HEPA filter
Autoclave

A pressure vessel that sterilizes equipment and waste using saturated steam under pressure at defined temperature and time, with air removal and load monitoring required for effectiveness.

Related: Sterilization, Biological indicator, Decontamination
Decontamination

A process that reduces or removes biological agents from surfaces, equipment, or people so that items become safe to handle, transport, or dispose of.

Related: Disinfection, Sterilization, Autoclave
Disinfection

A process that destroys or inactivates most recognized pathogenic microorganisms on inanimate objects, but does not necessarily destroy bacterial spores or all viruses.

Related: Decontamination, Sterilization, Sporicidal
Sterilization

A validated process that destroys or eliminates all forms of microbial life, including bacterial spores, and is verified by physical, chemical, and biological indicators.

Related: Autoclave, Disinfection, Biological indicator
Sporicidal

Describes a chemical or physical process able to kill bacterial endospores, the most resistant form of microbial life, under the labeled conditions of use.

Related: Disinfection, Sterilization
Biological indicator

A prepared population of resistant microorganisms, usually bacterial spores, used to demonstrate that a sterilization process achieved the required lethality.

Related: Autoclave, Sterilization
Sharps container

A rigid, leak-resistant, closable container used to collect needles, scalpels, and other sharp items so that handlers are protected from puncture injuries and contamination.

Related: Regulated medical waste, Biological waste
Biological waste

Material of biological origin or contaminated with biological agents that requires specific handling, treatment, and disposal to protect workers, the public, and the environment.

Related: Regulated medical waste, Sharps container, Autoclave
Regulated medical waste

A legal category of waste, defined by jurisdiction, that includes items such as blood-soaked materials, cultures, and sharps and must be segregated, treated, and tracked before disposal.

Related: Biological waste, Sharps container
Personal protective equipment

Clothing and devices worn by a worker, such as gloves, gowns, respirators, and eye protection, that form a barrier between the person and infectious or hazardous material.

Related: Donning and doffing, Respiratory protection
Donning and doffing

The ordered procedures for putting on and removing protective equipment so that contamination is not transferred to the wearer's skin, clothing, or clean areas during removal.

Related: Personal protective equipment, Respiratory protection
Respiratory protection

Equipment and programs, including fit testing, medical evaluation, and training, used to protect the wearer from inhaling airborne infectious agents or other hazardous particles.

Related: Personal protective equipment, HEPA filter
Standard precautions

A routine set of infection-control practices, including hand hygiene and barrier protection, applied to all patients and specimens regardless of known or suspected infection status.

Related: Personal protective equipment, Hand hygiene
Hand hygiene

Cleaning of hands by washing with soap and water or using an alcohol-based rub at defined moments, performed to break the chain of contact transmission.

Related: Standard precautions, Personal protective equipment
Aerosol

A suspension of fine solid or liquid particles in air, often generated by pipetting, centrifuging, or vortexing, that can carry infectious agents and be inhaled at a distance.

Related: Biological safety cabinet, Respiratory protection
Good microbiological practice

Basic safe handling habits, such as minimizing splashes and aerosols, keeping work areas clean, and washing hands, that reduce exposure during routine manipulation of cultures.

Related: Aerosol, Standard precautions
Spill response

A planned sequence of steps for containing, disinfecting, cleaning, and reporting a release of biological material, with actions scaled to the volume and hazard of the spill.

Related: Decontamination, Exposure control plan
Exposure control plan

A written program that identifies tasks with occupational exposure risk and specifies the engineering controls, work practices, protective equipment, and follow-up actions required to protect workers.

Related: Spill response, Post-exposure prophylaxis
Post-exposure prophylaxis

Medical treatment, such as vaccination or antimicrobial therapy, given after a documented exposure to reduce the chance of developing infection.

Related: Exposure control plan, Needlestick injury
Needlestick injury

A percutaneous injury caused by a contaminated sharp, which requires immediate care, source evaluation, and reporting so that appropriate follow-up can be started.

Related: Post-exposure prophylaxis, Sharps container
Biosecurity

Measures that prevent unauthorized access to, loss of, theft of, or misuse of biological agents and related information, complementing biosafety rather than replacing it.

Related: Risk assessment, Biosafety officer
Biosafety officer

A designated person responsible for advising on risk assessments, inspecting containment practices, investigating incidents, and maintaining the institution's biosafety program and records.

Related: Biosecurity, Biosafety program
Biosafety program

The organized policies, committees, training, inspections, and recordkeeping an institution uses to manage biological risks consistently across all laboratories and personnel.

Related: Biosafety officer, Competency-based training
Competency-based training

Instruction that pairs knowledge with observed, documented performance of a task, confirming that a person can safely carry out the procedure rather than only recall the rules.

Related: Biosafety program, Good microbiological practice
Certification

Formal verification, often by an outside tester, that a containment device or facility meets defined performance criteria and remains fit for use until the next scheduled test.

Related: Biological safety cabinet, Biosafety level
Material transfer agreement

A contract governing the shipment and use of biological materials between parties, specifying permitted use, handling requirements, and restrictions on further distribution.

Related: Biosecurity, Biological waste