MLS study plan
MLS · Give each week a clear focus.
8 weeks · About 8 hours per week
Blood Bank / Immunohematology
Map ABO and Rh antigen-antibody relationships, including weak and variant subgroups, by constructing a forward/reverse typing reference table
Resolve ABO typing discrepancies by working 12 self-authored case scenarios and writing the corrective step for each
Annotate three full 11-cell antibody panels to demonstrate rule-out, rule-in, dosage, and phase interpretation
Differentiate hemolytic, febrile non-hemolytic, allergic, septic, TRALI, and TACO reactions by tabulating onset, laboratory findings, and required workup
Justify the choice of immediate-spin, AHG, or electronic crossmatch for stated patient scenarios, and state the eligibility criteria for each
Your checkpoint
One-page decision flowchart for positive antibody screen through antigen-negative unit selection, plus 12 answered ABO discrepancy cases
Clinical Chemistry & Hematology and Hemostasis
Derive expected compensation for the four primary acid-base disorders using Winter's formula across eight blood gas cases
Calculate anion gap, osmolal gap, corrected sodium, corrected calcium, and eGFR, then flag artifact versus true abnormality in each
Correlate hepatic, renal, cardiac, and thyroid analyte panels with specific disease states in a comparative table
Build a CBC interpretation matrix that classifies anemia as microcytic, normocytic, or macrocytic using MCV, MCH, RDW, and reticulocyte response
Interpret PT/INR, aPTT, mixing studies, and factor assays to localize a coagulopathy to the intrinsic, extrinsic, or common pathway
Your checkpoint
A 25-question self-written chemistry and hematology quiz with worked rationales, self-scored
Microbiology & Blood Bank / Immunohematology
Classify bacteria by Gram reaction, morphology, oxidase, catalase, coagulase, and key biochemical reactions
Construct a specimen-to-pathogen table covering blood, CSF, urine, stool, wound, and respiratory sources with appropriate media and incubation conditions
Differentiate selective and differential media by purpose, and identify hemolysis patterns on blood agar
Interpret susceptibility results including MIC, disk diffusion zones, and resistance markers such as ESBL, MRSA, and CRE
Re-derive antigen frequency and serologic characteristics of 20 clinically significant alloantibodies to reinforce blood bank recall
Your checkpoint
A 40-organism identification grid plus a 10-case microbiology unknowns worksheet with answer key
Immunology and Serology & Urinalysis and Body Fluids
Compare EIA, ELISA, chemiluminescence, immunofluorescence, nephelometry, and immunodiffusion by principle, sensitivity, and common interference
Interpret ANA patterns and the syphilis, hepatitis, and HIV serologic testing algorithms step by step
Distinguish the four hypersensitivity types and primary versus secondary immunodeficiency using laboratory findings
Correlate urine dipstick chemistry with microscopic sediment findings and classify casts and crystals by clinical significance
Calculate and interpret CSF-to-serum glucose and protein ratios, cell counts, and body fluid quality-control checks
Your checkpoint
A two-page serology algorithm reference plus a 20-item urinalysis and body fluid case set with written answers
Molecular Diagnostics & Laboratory Operations and Management
Trace molecular workflows from extraction through amplification, detection, and contamination control for PCR, RT-PCR, TMA, and isothermal methods
Differentiate hybridization, Sanger sequencing, next-generation sequencing, blotting, and microarray techniques by application and limitation
Interpret molecular results including Ct values, viral load trends, genotypes, and mutation panels
Apply Levey-Jennings charts and Westgard rules to classify random and systematic error in given QC data
Classify CLIA test complexity categories, CAP accreditation requirements, and OSHA safety obligations for a given laboratory scenario
Your checkpoint
A QC shift-and-rule decision sheet plus a molecular method-selection table with at least 10 assay-to-application pairings
Blood Bank / Immunohematology & Hematology and Hemostasis
Re-solve 20 antibody identification cases under timed conditions and log every miss by failure mode
Re-derive the laboratory workup for DIC, heparin-induced thrombocytopenia, von Willebrand disease, hemophilia, and heparin monitoring
Recompute RBC indices and match peripheral smear descriptions to 15 specific hematologic disorders
Verify transfusion service quality control requirements for antisera, antiglobulin reagents, and check cells
Your checkpoint
An error log of at least 20 missed items, each with a written root cause and a concrete correction strategy
Clinical Chemistry & Microbiology
Re-solve 25 timed chemistry calculation and case-based questions, targeting previously missed categories
Re-identify 30 organisms using only biochemical flowcharts and colony characteristics
Compile a rapid-reference set covering ova and parasites, CSF microbiology, and stool culture screening
Diagnose simulated chemistry and microbiology quality-control failures and state the corrective action for each
Your checkpoint
A one-page must-know numbers reference card for clinical chemistry plus a 30-organism rapid identification flowchart
Urinalysis and Body Fluids & Laboratory Operations and Management
Complete a full-length timed mock exam of 200 questions spanning all eight domains
Grade the exam item by item, tagging every miss by domain and error type
Re-teach the three most frequent recurring error patterns identified in the tagging step
Review urinalysis and body fluid edge cases and laboratory operations math and regulatory items before test day
Your checkpoint
A scored full-length mock exam with a written remediation plan listing the top 10 remaining knowledge gaps and the action for each
Milestones
Week 2: Score at least 70% on the 25-item mixed chemistry and hematology quiz, and correctly resolve at least 8 of 12 ABO discrepancy cases
Week 4: Complete the serology algorithm sheet and score at least 75% on a cumulative 60-question quiz covering domains 1 through 4
Week 6: Achieve at least 80% on timed antibody identification and hemostasis sets, with an error log containing 20 or more documented entries
Week 8: Score at least 75% on the full-length mock exam with no single domain falling below 65%