MLS study materials
MLS study resources

MLS study plan

MLS · Give each week a clear focus.

8 weeks · About 8 hours per week

Week 1

Blood Bank / Immunohematology

  • Map ABO and Rh antigen-antibody relationships, including weak and variant subgroups, by constructing a forward/reverse typing reference table

  • Resolve ABO typing discrepancies by working 12 self-authored case scenarios and writing the corrective step for each

  • Annotate three full 11-cell antibody panels to demonstrate rule-out, rule-in, dosage, and phase interpretation

  • Differentiate hemolytic, febrile non-hemolytic, allergic, septic, TRALI, and TACO reactions by tabulating onset, laboratory findings, and required workup

  • Justify the choice of immediate-spin, AHG, or electronic crossmatch for stated patient scenarios, and state the eligibility criteria for each

Your checkpoint

One-page decision flowchart for positive antibody screen through antigen-negative unit selection, plus 12 answered ABO discrepancy cases

Week 2

Clinical Chemistry & Hematology and Hemostasis

  • Derive expected compensation for the four primary acid-base disorders using Winter's formula across eight blood gas cases

  • Calculate anion gap, osmolal gap, corrected sodium, corrected calcium, and eGFR, then flag artifact versus true abnormality in each

  • Correlate hepatic, renal, cardiac, and thyroid analyte panels with specific disease states in a comparative table

  • Build a CBC interpretation matrix that classifies anemia as microcytic, normocytic, or macrocytic using MCV, MCH, RDW, and reticulocyte response

  • Interpret PT/INR, aPTT, mixing studies, and factor assays to localize a coagulopathy to the intrinsic, extrinsic, or common pathway

Your checkpoint

A 25-question self-written chemistry and hematology quiz with worked rationales, self-scored

Week 3

Microbiology & Blood Bank / Immunohematology

  • Classify bacteria by Gram reaction, morphology, oxidase, catalase, coagulase, and key biochemical reactions

  • Construct a specimen-to-pathogen table covering blood, CSF, urine, stool, wound, and respiratory sources with appropriate media and incubation conditions

  • Differentiate selective and differential media by purpose, and identify hemolysis patterns on blood agar

  • Interpret susceptibility results including MIC, disk diffusion zones, and resistance markers such as ESBL, MRSA, and CRE

  • Re-derive antigen frequency and serologic characteristics of 20 clinically significant alloantibodies to reinforce blood bank recall

Your checkpoint

A 40-organism identification grid plus a 10-case microbiology unknowns worksheet with answer key

Week 4

Immunology and Serology & Urinalysis and Body Fluids

  • Compare EIA, ELISA, chemiluminescence, immunofluorescence, nephelometry, and immunodiffusion by principle, sensitivity, and common interference

  • Interpret ANA patterns and the syphilis, hepatitis, and HIV serologic testing algorithms step by step

  • Distinguish the four hypersensitivity types and primary versus secondary immunodeficiency using laboratory findings

  • Correlate urine dipstick chemistry with microscopic sediment findings and classify casts and crystals by clinical significance

  • Calculate and interpret CSF-to-serum glucose and protein ratios, cell counts, and body fluid quality-control checks

Your checkpoint

A two-page serology algorithm reference plus a 20-item urinalysis and body fluid case set with written answers

Week 5

Molecular Diagnostics & Laboratory Operations and Management

  • Trace molecular workflows from extraction through amplification, detection, and contamination control for PCR, RT-PCR, TMA, and isothermal methods

  • Differentiate hybridization, Sanger sequencing, next-generation sequencing, blotting, and microarray techniques by application and limitation

  • Interpret molecular results including Ct values, viral load trends, genotypes, and mutation panels

  • Apply Levey-Jennings charts and Westgard rules to classify random and systematic error in given QC data

  • Classify CLIA test complexity categories, CAP accreditation requirements, and OSHA safety obligations for a given laboratory scenario

Your checkpoint

A QC shift-and-rule decision sheet plus a molecular method-selection table with at least 10 assay-to-application pairings

Week 6

Blood Bank / Immunohematology & Hematology and Hemostasis

  • Re-solve 20 antibody identification cases under timed conditions and log every miss by failure mode

  • Re-derive the laboratory workup for DIC, heparin-induced thrombocytopenia, von Willebrand disease, hemophilia, and heparin monitoring

  • Recompute RBC indices and match peripheral smear descriptions to 15 specific hematologic disorders

  • Verify transfusion service quality control requirements for antisera, antiglobulin reagents, and check cells

Your checkpoint

An error log of at least 20 missed items, each with a written root cause and a concrete correction strategy

Week 7

Clinical Chemistry & Microbiology

  • Re-solve 25 timed chemistry calculation and case-based questions, targeting previously missed categories

  • Re-identify 30 organisms using only biochemical flowcharts and colony characteristics

  • Compile a rapid-reference set covering ova and parasites, CSF microbiology, and stool culture screening

  • Diagnose simulated chemistry and microbiology quality-control failures and state the corrective action for each

Your checkpoint

A one-page must-know numbers reference card for clinical chemistry plus a 30-organism rapid identification flowchart

Week 8

Urinalysis and Body Fluids & Laboratory Operations and Management

  • Complete a full-length timed mock exam of 200 questions spanning all eight domains

  • Grade the exam item by item, tagging every miss by domain and error type

  • Re-teach the three most frequent recurring error patterns identified in the tagging step

  • Review urinalysis and body fluid edge cases and laboratory operations math and regulatory items before test day

Your checkpoint

A scored full-length mock exam with a written remediation plan listing the top 10 remaining knowledge gaps and the action for each

Milestones

Week 2: Score at least 70% on the 25-item mixed chemistry and hematology quiz, and correctly resolve at least 8 of 12 ABO discrepancy cases

Week 4: Complete the serology algorithm sheet and score at least 75% on a cumulative 60-question quiz covering domains 1 through 4

Week 6: Achieve at least 80% on timed antibody identification and hemostasis sets, with an error log containing 20 or more documented entries

Week 8: Score at least 75% on the full-length mock exam with no single domain falling below 65%