CI-CPT study materials
CI-CPT study resources

CI-CPT key terms

CI-CPT · Get comfortable with the vocabulary.

Order of draw

The standardized sequence in which blood collection tubes are filled to prevent additive carryover between tubes. It typically begins with blood culture bottles, then coagulation tubes, serum tubes, heparin tubes, and ends with tubes containing EDTA or glycolytic inhibitors.

Related: Additive carryover, Clot activator, Evacuated tube system
Additive carryover

Transfer of an additive such as EDTA or heparin from one tube into the next through a needle or holder, potentially altering test results. Proper order of draw and discarding the first drop minimize this contamination risk.

Related: Order of draw, Evacuated tube system
Evacuated tube system

A closed collection set consisting of a double-pointed needle, a plastic holder, and vacuum-sealed tubes that draw a predetermined blood volume. It reduces exposure risk and improves specimen quality compared with open systems.

Related: Order of draw, Multi-sample needle, Hemolysis
Multi-sample needle

A double-ended needle with a beveled patient end and a shorter rubber-sheathed end that punctures collection tubes. The sleeve reseals after each tube is removed so multiple tubes can be filled from one venipuncture.

Related: Evacuated tube system, Butterfly needle
Butterfly needle

A winged infusion set with flexible tubing attached to a short needle, used for fragile or small veins and for pediatric patients. Its extra dead space makes it unsuitable for collecting coagulation specimens unless a discard tube is drawn first.

Related: Multi-sample needle, Order of draw
Capillary puncture

Collection of blood from a skin nick, usually on a fingertip or infant heel, used when venipuncture is impractical or a small volume suffices. Warming the site increases arterial flow and improves yield.

Related: Lancet depth, Hemolysis
Lancet depth

The penetration distance of a puncture device into the skin. Depth must be chosen by site and patient age; heel sticks in infants are limited to about 2 mm to avoid striking the calcaneus and causing osteomyelitis.

Related: Capillary puncture, Hemolysis
Tourniquet time

The interval a constricting band remains applied above the puncture site. It should not exceed about one minute, because prolonged stasis causes hemoconcentration and falsely elevates potassium, protein, and cell counts.

Related: Hemoconcentration, Venipuncture site selection
Venipuncture site selection

Choosing a vein suitable for puncture by palpation, generally the median cubital or cephalic vein in the antecubital fossa. Areas with scarring, hematoma, intravenous lines, or dialysis fistulas are avoided.

Related: Tourniquet time, Hemoconcentration
Hemoconcentration

A relative increase in red cells and large molecules caused by fluid shifting out of the vessels, often from prolonged tourniquet application or dehydration. It can falsely raise hemoglobin, potassium, and protein results.

Related: Tourniquet time, Hemolysis
Hemolysis

Rupture of red blood cells that releases hemoglobin and intracellular contents into the plasma or serum. It falsely elevates potassium, lactate dehydrogenase, and AST and can interfere with many other assays.

Related: Specimen rejection criteria, Centrifugation, Hemoconcentration
Clot activator

A substance such as silica or thrombin coated on the inner wall of a serum tube to speed and complete coagulation. Complete clotting before centrifugation is essential to avoid fibrin strands in the separated serum.

Related: Serum separator tube, Order of draw
Serum separator tube

A collection tube containing clot activator and a thixotropic gel that forms a barrier between serum and cells during centrifugation. Proper clotting time before spinning prevents latent fibrin formation.

Related: Clot activator, Centrifugation
Centrifugation

The spinning of specimens at a defined relative centrifugal force and time to separate cells from plasma or serum. Correct speed, duration, and balanced loading are required to avoid hemolysis and incomplete separation.

Related: Hemolysis, Serum separator tube
Specimen rejection criteria

Predefined conditions under which a laboratory refuses to test a sample, such as unlabeled tubes, gross hemolysis, insufficient volume, or improper transport temperature. Rejection protects patient safety and result validity.

Related: Hemolysis, Specimen labeling, Chain of custody
Specimen labeling

Applying patient identifiers to a container at the bedside immediately after collection. Most facilities require two independent identifiers and labeler initials; unlabeled or mislabeled tubes must be recollected, not corrected.

Related: Specimen rejection criteria, Patient identification
Chain of custody

A documented, unbroken record of who handled a specimen and when, required for forensic, employment, and legal drug testing. Each transfer is signed and timed so the sample's integrity can be defended.

Related: Specimen rejection criteria, Specimen labeling
Patient identification

Verification of identity using at least two independent identifiers, such as full name and date of birth, plus a verbal confirmation when possible. It is performed before every collection and before any point-of-care test.

Related: Specimen labeling, Point-of-care testing
Informed consent

A patient's voluntary agreement to a procedure after receiving an explanation of its purpose, risks, and alternatives. Consent may be verbal for routine draws but must be documented for research or special procedures.

Related: Patient identification, Needlestick exposure
Needlestick exposure

An accidental percutaneous injury from a contaminated needle or sharp. Immediate washing, reporting, and post-exposure prophylaxis evaluation are required because of bloodborne pathogen transmission risk.

Related: Standard precautions, Sharps container
Standard precautions

Infection control practices applied to all patients regardless of diagnosis, including hand hygiene, gloves, and eye protection when splash risk exists. They treat all blood and body fluids as potentially infectious.

Related: Needlestick exposure, Sharps container
Sharps container

A puncture-resistant, leak-proof, labeled receptacle for used needles, lancets, and other contaminated sharps. It is replaced when about three-quarters full and never overfilled or shaken.

Related: Needlestick exposure, Standard precautions
Syncope

A transient loss of consciousness from reduced cerebral blood flow, often triggered by the sight of blood or needle anxiety. Patients should be positioned safely and monitored until fully recovered.

Related: Patient identification, Informed consent
Point-of-care testing

Diagnostic testing performed near the patient rather than in a central laboratory, using devices such as glucose meters or blood gas analyzers. Results are immediate but require operator competency and quality control.

Related: Quality control, Capillary puncture, Patient identification
Quality control

Scheduled testing of control materials with known values to verify that an instrument or test system is performing within acceptable limits. Results are documented before patient samples are reported.

Related: Point-of-care testing, Proficiency testing
Proficiency testing

Periodic evaluation in which a laboratory or testing site analyzes unknown specimens supplied by an external program and compares results with peer laboratories. It is a required component of accreditation and regulatory compliance.

Related: Quality control, Point-of-care testing
Critical value

A result so far outside the reference range that it may indicate imminent danger to the patient. It must be reported promptly to a responsible caregiver and the notification documented.

Related: Point-of-care testing, Quality control
Delta check

Comparison of a current result with the patient's previous result to detect a significant change that may signal specimen error or a true clinical event. It is one safeguard against reporting wrong-patient results.

Related: Critical value, Specimen rejection criteria
Health Insurance Portability and Accountability Act

United States federal law that protects the privacy and security of individually identifiable health information. It limits disclosure of patient data and requires safeguards in both paper and electronic records.

Related: Confidentiality, Informed consent
Confidentiality

The ethical and legal duty to keep patient information private and share it only with those who need it for care. It applies to conversations, records, and test results in all settings.

Related: Health Insurance Portability and Accountability Act, Professional boundaries
Professional boundaries

The appropriate limits of the relationship between a healthcare worker and a patient, preserving objectivity and avoiding personal or financial entanglement. Boundary crossings should be recognized and reported.

Related: Confidentiality, Informed consent
Therapeutic communication

Purposeful verbal and nonverbal techniques, such as open-ended questions, reflection, and active listening, that build rapport and reduce patient anxiety. It avoids jargon, judgment, and false reassurance.

Related: Informed consent, Professional boundaries
Scope of practice

The tasks and responsibilities a healthcare worker is legally and professionally authorized to perform based on training, licensure, and employer policy. Performing outside it creates liability and patient risk.

Related: Professional boundaries, Point-of-care testing
Specimen transport temperature

The required thermal condition for moving a sample from collection site to laboratory, such as ambient, refrigerated, or frozen. Deviation can invalidate analytes like ammonia or blood gases.

Related: Specimen rejection criteria, Centrifugation
Blood culture collection

Obtaining blood for microbial culture using aseptic skin preparation and bottles drawn before other tubes. Volume and the number of sets strongly influence sensitivity for detecting bacteremia.

Related: Order of draw, Standard precautions