GATE BM case studies
GATE BM · Put your knowledge to work in context.
GATE - the Graduate Aptitude Test in Engineering - is a national-level examination that primarily tests comprehensive understanding of undergraduate subjects in Engineering, Technology, Architecture, Science, Commerce, Arts and Humanities. For 2025 the organising institute is IIT Roorkee, and the exam is conducted by IISc Bengaluru together with seven IITs (Bombay, Delhi, Guwahati, Kanpur, Kharagpur, Madras and Roorkee) on behalf of the National Coordination Board – GATE, Department of Higher Education, Ministry of Education, Government of India. The GATE BM case studies are 5 cases and about 3,642 words.
1Off-gas RQ anomaly in an aerobic yeast fed-batch
An 800 L fed-batch culture of recombinant Saccharomyces cerevisiae is aerated at 0.5 vvm with air (20.9% O2, 0.04% CO2). Off-gas analysis at steady state reads 19.0% O2 and 2.50% CO2.
768 words, about 4 min2Protein A capture shortfall in a CHO antibody batch
A 2,000 L CHO fed-batch harvest contains a monoclonal antibody at 1.20 g/L. The capture step uses a 40 L Protein A column with a dynamic binding capacity of 35 g/L; it is run in two cycles.
661 words, about 3 min3Multiplex RT-qPCR inhibition and target dropout
A research laboratory is validating a two-target RT-qPCR assay for an enveloped RNA virus in nasopharyngeal swabs. Extraction is automated in 96-well plates with an exogenous RNase P internal control (IC) added before lysis.
704 words, about 4 min4Choosing T1 progeny after CRISPR editing in rice
Rice transformation produced 150 independent T0 lines carrying Cas9, an sgRNA targeting OsGENE, and a hygromycin resistance cassette. Deep amplicon sequencing of T0 leaf DNA gives: line A, 62% reads with a 1-bp insertion and 5% wild type; line B, 96% reads with a 4-bp deletion; line C, 34% edited reads with three different indels; line D, no editing.
737 words, about 4 min5Packed-bed immobilized enzyme scale-up with mass-transfer limitation
A packed-bed reactor is being designed for enzymatic hydrolysis of a soluble substrate. The enzyme is immobilized on 200 micrometre porous beads at 20 g/L reactor volume.
772 words, about 4 min