JBA Written Exam case studies
JBA Written Exam · Put your knowledge to work in context.
The JBA Written Exam is the written assessment associated with the Japan Bioindustry Association's biosafety and bioindustry credentialing programme. It is aimed at people who work, or intend to work, in laboratories, production facilities and quality units where biological materials are handled: biosafety officers, facility and equipment managers, QA staff, quality control analysts, and researchers moving into compliance roles. The JBA Written Exam case studies are 5 cases and about 4,388 words.
1Pressure Reversal in a BSL-3 Suite at 02:40
A university BSL-3 suite has a service corridor, an anteroom and a work room, validated at corridor 0 Pa, anteroom -30 Pa and work room -50 Pa. At 02:40 the building management system alarms: the anteroom now reads +6 Pa relative to the corridor and the work room has drifted to -12 Pa.
948 words, about 5 min2Cracked Tube in a Non-Aerosol-Tight Rotor: Concentrated Lentiviral Vector
A neuroscience group packages third-generation, self-inactivating lentiviral vectors carrying an shRNA at 1 x 10^8 transducing units/mL. On Tuesday a technician pools 40 mL of vector-containing supernatant into a 50 mL polycarbonate Oak Ridge tube and centrifuges it at 4,000 x g for 20 minutes in a benchtop rotor whose aerosol-tight lid gasket has been visibly worn for months.
859 words, about 4 min3Mycoplasma Signal and an Extra STR Peak in a Three-Year-Old Master Cell Bank
A 200-vial CHO-derived master cell bank (MCB) was manufactured three years ago and is stored in a liquid-nitrogen tank that also holds a partner company's MCB on the same rack. Eight months ago the tank's level alarm sounded and the automatic fill failed for about 60 hours before manual filling; no inventory was lost and the event was closed.
862 words, about 4 min4Three Positive Units in a 5,000-Unit Media Fill, Confirmed After Twelve Batches Were Released
An aseptic filling line fills 2 mL vials on a six-head peristaltic filler inside an ISO 5 (Grade A) critical zone with an ISO 7 (Grade B) background. The latest aseptic process simulation (media fill) ran 5,000 units over two shifts and gave three positive units, all from head 4, two of them in the final hour.
886 words, about 4 min5A Cross-Border Shipment of a 'Disabled' Recombinant Strain
A company produces a recombinant Escherichia coli K-12 strain (kanamycin-resistant, delta-thyA, carrying a plasmid expressing a non-toxic industrial enzyme) for a customer developing an enzyme process. The customer asks for 20 research vials to be shipped to its own laboratory in another country where the strain is not yet authorised for use.
833 words, about 4 min