BSEH SS Biotechnology case studies
BSEH SS Biotechnology · Put your knowledge to work in context.
The BSEH Senior Secondary Biotechnology course is the Class 12 elective offered under the Board of School Education Haryana, and the board examination is what a candidate typically means by "BSEH SS Biotechnology." It suits students who have finished Class 11 science and want a subject that sits between pure biology and applied laboratory work - useful for those heading toward B.Sc. Biotechnology, microbiology, pharmacy, agriculture, or paramedical courses, and for anyone who wants a practical science subject alongside Physics and Chemistry. The BSEH SS Biotechnology case studies are 5 cases and about 4,169 words.
1The internal NdeI site that broke the lipase clone
An MSc student has amplified the 1,842 bp open reading frame of a bacterial lipase by PCR with primers designed to add NdeI and XhoI sites, intending to clone it into pET-28a(+) cut with the same enzymes so that the protein carries an N-terminal His6 tag. Ligation, transformation into BL21(DE3) and selection on kanamycin give 60 colonies.
751 words, about 4 min2Bulk starter collapses: phage or residue?
An independent dairy produces 5,000 L of set yogurt per day in two 1,000 L tanks, using a bulk starter of Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus grown in 10% reconstituted skim milk.
706 words, about 4 min3Glassiness and a slow white contamination in banana micropropagation
A commercial banana (Musa AAA 'Grand Naine') micropropagation laboratory produces 20,000 plantlets per month from shoot-tip cultures on MS medium with 3% sucrose, 1.0 mg/L BAP, 0.5 mg/L IAA, 0.8% agar, pH 5.8, in 250 mL jars with vented lids, at 25 °C and a 16 h photoperiod. To cut costs three weeks ago, agar was reduced to 0.55%, lids were sealed with Parafilm instead of vented tape, and light intensity was halved.
665 words, about 3 min4A reactive HIV combo assay with a negative NAT in a repeat blood donor
A blood bank tests 80 donations daily with a fourth-generation HIV-1/2 antigen–antibody combination ELISA and, for reactive units, an individual-donation nucleic acid test (ID-NAT) for HIV, HCV and HBV. A 26-year-old repeat donor's donation is reactive with an S/CO of 4.8 on the combination assay; the ID-NAT is negative and a point-of-care rapid immunochromatographic test is also negative.
951 words, about 5 min5A patent on a peptide found under a research-only permit
A university team constructs a metagenomic library from 40 soil samples collected in a state biosphere reserve under a permit stating 'research use only; no commercial exploitation; no transfer of biological material to third parties without written permission from the Chief Wildlife Warden'. From the library they identify a 34-amino-acid lantibiotic active at 0.5 µg/mL against vancomycin-resistant Enterococcus.
1,096 words, about 5 min